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How can you set up a bacteria culture in a school lab?
Get agar jelly and insert your penis into the culture.
Why is agar special in microbiology?
agar is used for growing microbes as to culture microorganisms you must provide a culture medium containing carbohydrate as an energy source along with mineral ions and protein and vitamins. these nutrients are often contained in an agar medium. agar is a substance that can dissolve in hot water to form a jelly. you pour hot agar into a petri dish to set as a way to provide all the nutrients for the microbes to reproduce successfully. AGAR helps to make the media in semisolid or in jelly form which allow the microbial colonies to grow on surface by providing them better surface and we can study the colonial character of different species of microbes.
How do you isolate pure culture from the mouth?
To isolate a pure culture from the mouth, a sample can be collected and streaked onto a selective agar medium, such as blood agar, that inhibits the growth of unwanted bacteria. After incubation, individual colonies can be picked and streaked onto a fresh plate multiple times to obtain a pure culture. Various biochemical tests can be conducted to further confirm the identity of the isolated bacterium.
How fast can bacteria species grow on agar plates?
ita taka lika tree yeara to growa lika 24 hrsa
What is a depression plate in chemistry?
A depression plate in chemistry is a type of plate set aside for a chemical reaction to occur. It can be used for items involving chemical salts or kinetics.
How can you set up a bacteria culture in a school lab?
Get agar jelly and insert your penis into the culture.
Why are agar plates dried before being used for the preparation of dilution streak plates?
perhaps it is easier to streak that way, i mean when the agar is set and dry. .
Use of agar slant?
An agar slant is when a test tube is filled with liquid agar and allowed to cool and harden at an angle (slant). Agar is mixed with other nutrients to provide a medium for which bacteria can grow on.
Why is agar special in microbiology?
agar is used for growing microbes as to culture microorganisms you must provide a culture medium containing carbohydrate as an energy source along with mineral ions and protein and vitamins. these nutrients are often contained in an agar medium. agar is a substance that can dissolve in hot water to form a jelly. you pour hot agar into a petri dish to set as a way to provide all the nutrients for the microbes to reproduce successfully. AGAR helps to make the media in semisolid or in jelly form which allow the microbial colonies to grow on surface by providing them better surface and we can study the colonial character of different species of microbes.
How do the results of pour plate method compare with those obtained using the streak plate and spread plate method?
In the pour plate, the microorganisms will grow within the gel that has been set, and in the spread-plate technique, growth will be on top of the agar gel where it has been spread.
What are the differences between streak plate technique and pour plate technique?
Streak Plate:Pure colonies of bacterial or other microorganisms can be obtained on petri dishes by streak plate. The microbial mixture is transfered to the edge of an agar plate with an inoculating loop or swab and then streaked out over the surface in one of several patterns. After the first sector is streaked in dish, the inoculating loop is sterlized and an inoculum for the second sector is obtained from the first sector. The same is done for third and fourth sector. Thus this is a dilution process. Eventually, very few cells will be on inoculating loop, a single cells will drop from it as it is rubbed along the agar surface. These develop into seprate colonies.Pour Plate:Extensively used with procaryotes and fungi, a pour plate also can yield isolated colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating. Then small volumes of several diluted samples are mixed with liquid agar that has been cooled to about 45oC and the mixture are poured immediately into sterile culture dishes. Most bacteria and fungi are not killed by a brief exposure to the warm agar. After the agar has hardened each cell is fixed in place and forms an individual colony.
How do you isolate pure culture from the mouth?
To isolate a pure culture from the mouth, a sample can be collected and streaked onto a selective agar medium, such as blood agar, that inhibits the growth of unwanted bacteria. After incubation, individual colonies can be picked and streaked onto a fresh plate multiple times to obtain a pure culture. Various biochemical tests can be conducted to further confirm the identity of the isolated bacterium.
When do you take the ames test?
The ames test is a basic toxicological test that can be used to determine if a substance is potentially genotoxic. It is a very easy and cheap test that can be set up in most labs. The test looks for gene mutations in bacteria, normally Salmonella typhimurium. A strain of S. typhimurium with a mutation that causes it to be unable to synthezise histidine (amino acid) is plated along with the substance to be tested. The mutation in the bacteria can easily be backmutated, thus, any substance with a genotoxic (mutagenic) ability can cause the bacteria to backmutate to a state where it can again syntesize histidine. S. typh on agar plate without histidine will not be able to grow, and thus, no colony forming units (cfu) will be seen. S. typh on agar plate, add potentially genotoxic substance onto agar (normally on paper disc), the substance will filter through agar, and if mutagenic, can cause backmutation in the bacteria which will then be able to synthesize histidine, resulting in growt of cfu's. The results seen in the ames test can give an idea of the substances potential for genotoxicity. If the substance has the ability to cause a mutation in the bacteria, it might be able to cause mutations in humans as well, and should therefore undergo further testing to determine whether it is safe or not.
What is meant by streak dilution technique?
The streak plate technique is a method of diluting bacteria down suficiently so that the will grow as single colonies. The technique varies from individual to individual so much so that you can identify a researcher's plates much like their handwritting! The technique is somewhat more standardised in hospital labs and a printed out sheet is placed below the plate for the operative to follow as a guide. The technique is usually taught like this; 1) Flame your loop and aseptically take 1 loopful of culture and place it a 12 o'clock on your plate draw a straight line 5cm across the plate ending around 2.30o'clock. 2) Lift the loop and draw two more lines parallel the first about 0.5 cm distance below the first. 3) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines over lapping the first set. (your end at 5o'clock) 4) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the second set. (you end at 6.30o'clock) 5)Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the third set. (your end at 8o'clock) 6) Flame your loop this time instead of a set of lines start by overlapping the fourth set of lines and then draw a scribble into the middle of your plate using as much of the unused agar as possible. The technique is sort of a dilution becasue each time you flame your loop it is sterilised, when you then draw out some of the bacteria from your last set of lines and spread them over a much greater area.
How fast can bacteria species grow on agar plates?
ita taka lika tree yeara to growa lika 24 hrsa
When was 'In' Jazz for the Culture Set created?
'In' Jazz for the Culture Set was created in 1965.
What is the purpose of motility agar?
Motility Test Agar is used for the differentiation of microorganisms on the basis of motility.Many bacteria have flagella that make them capable of swimming through water-based environments. Motility can be determined by observing cells in a wet mount. However this determination can be difficult because bacteria are small enough that they are bounced around by water molecules. This random movement, called Brownian motion, can be confused with self-propelled motility. Another way to determine motility is with a Motility Agar stab. Motility Agar is soft agar in a test tube (without a slanted surface). Cells are stab-inoculated into the agar (the top surface is not inoculated). Non-motile bacteria will only grow where they were inoculated. Motile bacteria will grow along the stab and will also swim out away from the stabbed area. Thus, a negative result is indicated by growth in a distinct zone directly along the stab. A positive result is indicated by diffuse (cloudy growth), especially at the top and bottom of the stab. Results {| ! scope="col" | + - Positive (cloudy, diffuse growth)! scope="row" | - = Negative (well-defined growth along the stab).! scope="row" | V = Variable! scope="row" |! scope="row" || CAUTION: This test only works with organisms that can grow anaerobically (e.g., facultative anaerobes). Obligately aerobic organisms will grow on the top of the agar but will not grow in the stab.|}
