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There are the 5 “I’s”
1. Inoculate
2. Incubate
3. Isolation
4. Inspection
5. Identification
1. Take a sample from the mouth using aseptic techniques (meaning don't cross contaminate with you own bacteria).
2. Inoculate a medium of some sort. Usually a plate called a streak plate is used. It has nutrient agar in it. It looks a little like vey stiff Jell-O. Spread the sample using a loop or 'hockey stick' using aseptic techniques.
3. Let it grow in an incubator. Set (usually) at body temperature. The plate is always placed what you would call upside down. Leave it there for 24-36 hours.
4. Open plate (aseptically) and see what is growing there. If something has you attention, use a probe (aseptically) to take a bit and put into a growth medium (usually in this case a liquid). Leave for a time and then replate.
5. The ID part means using a microscope to see the basic shapes, and also using differential media to further ID. This can mean reincubation and can take several days.
Wiki User
∙ 7y ago
To isolate a pure culture from the mouth, a sample can be collected and streaked onto a selective agar medium, such as blood agar, that inhibits the growth of unwanted bacteria. After incubation, individual colonies can be picked and streaked onto a fresh plate multiple times to obtain a pure culture. Various biochemical tests can be conducted to further confirm the identity of the isolated bacterium.
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Why is it necessary to isolate individualcolonies from a mixed growth?
In order to identify any of the species in a mixed culture, you first have to isolate individual colonies and grow them in a pure culture. You can't perform tests to identify bacteria in a mixed culture.
Why obtain pure culture of bacteria from a mixed culture?
i obtain pure culture of bacteria from a mixed culture for obtain pure one bacterial culture
Why dilution is a common approach employed by any pure culture technique?
the main purpose of this is to grow and isolate all bacteria present in an infection, to determine which of the bacteria that grew are most likely causing the infection and which are likely colonizers.
Is pure culture having same meaning of microbial culture?
A pure culture is a microbial culture of only one organism. A microbial culture could contain any number of organisms.
Why is dilutions a necessary part of pure culture preparation?
Dilution is necessary for getting isolated colonies, from which pure culture shall be obtained for further study.
What is the purpose of quadrant streaking in bacterial cultivation?
The purpose is to isolate a pure culture of bacteria from a mixed culture of bacteria.
Why is it necessary to isolate individualcolonies from a mixed growth?
In order to identify any of the species in a mixed culture, you first have to isolate individual colonies and grow them in a pure culture. You can't perform tests to identify bacteria in a mixed culture.
Can streak or spread plates isolate pure cultures from mixed bacteria?
YES
Could you distinguish between axenic culture and pure culture?
a pure culture is the one that originate from a single cell and has same specie of an organism while axenic culture can originate from more than one cell.
Why obtain pure culture of bacteria from a mixed culture?
i obtain pure culture of bacteria from a mixed culture for obtain pure one bacterial culture
What is the difference between pure culture and auxanic culture?
A pure culture is one in which only one kind of microbial species is found whereas in mixed culture two or more microbial species formed colonies.
Is pure culture having same meaning of microbial culture?
A pure culture is a microbial culture of only one organism. A microbial culture could contain any number of organisms.
What are the prevention pure culture contamination?
if contamination is noted in a subculture sample, what should be to obtain a pure culture
Why dilution is a common approach employed by any pure culture technique?
the main purpose of this is to grow and isolate all bacteria present in an infection, to determine which of the bacteria that grew are most likely causing the infection and which are likely colonizers.
What is the difference between 100 whey protein and pure whey isolate?
whey protein(concentrate or 100%) contains usually between 70 to 80% protein while isolate 90% or more....
Why is dilutions a necessary part of pure culture preparation?
Dilution is necessary for getting isolated colonies, from which pure culture shall be obtained for further study.
How would you define pure culture sterile and contamination in the isolation and purification of bacteria?
A pure culture is defined as a population containing only a single species or strain of bacteria. Contamination means that more than one species is present in a culture that is supposed to be pure.
