hi, could any one answer what is the difference between hplc uv detector and spectrophotometer uv detector ? my id is debendra_mallick2002@yahoomail.com
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
flourescence is more sensetive than UV detection
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
by injecting the sample in to a HPLC system having PDA detector or Scan the Quetiapine Fumarate sample solution in UV range.
hi, could any one answer what is the difference between hplc uv detector and spectrophotometer uv detector ? my id is debendra_mallick2002@yahoomail.com
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
flourescence is more sensetive than UV detection
We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
Of course.... there isn´t problem....
1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution
by injecting the sample in to a HPLC system having PDA detector or Scan the Quetiapine Fumarate sample solution in UV range.
A cobra detector is a radar detector. Radar detectors work the same way a radio receiver does. The radar detector looks for frequencies that a radar gun uses.
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
HPLC is advanced