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Q: How does an RID detector work for HPLC?
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What is Difference Between HPLC UV detector Spectrophotometer uv detector?

hi, could any one answer what is the difference between hplc uv detector and spectrophotometer uv detector ? my id is debendra_mallick2002@yahoomail.com


Definition of standard in HPLC?

Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.


What is difference between an HPLC UV detector and a fluorescence detector?

flourescence is more sensetive than UV detection


How you can analyze pregabalin on HPLC with UV detector?

We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.


Why is Erbium perchlorate is used in calibration of HPLC?

Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.


Can you use a C18 column for HPLC with fluorescence detector and methanol as the mobile phase?

Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.


Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?

Of course.... there isn´t problem....


What are the HPLC Calibration parameters and elaborate it?

1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution


How Lambda max determined for quetiapine fumarate?

by injecting the sample in to a HPLC system having PDA detector or Scan the Quetiapine Fumarate sample solution in UV range.


How does a cobra detector work?

A cobra detector is a radar detector. Radar detectors work the same way a radio receiver does. The radar detector looks for frequencies that a radar gun uses.


How do you distinguised np-hplc and rp-hplc?

NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).


Which one is advanced gc or hplc?

HPLC is advanced