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Glycerol replace water. Water can damage because it crystallize when lower 0 C. And also bi-layer membranes is supported in liquid state by it.
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Can you preserve or freeze a bacterial isolate in a normal freezer by just growing them in a broth with glycerol?
Add the sterile glycerol after growing them in broth. However, -80 freezer preserves them for longer than normal freezer.
Where to find non pathogenic freeze dried staph bacteria with growth medium?
Freeze-dried staphylococcus epidermis bacteria (non-pathogenic) where to buy
Can you freeze chicken twice?
No, you can not freeze chicken twice. Refreezing meats can cause bacteria to start growing and will make you sick when you eat the chicken.
Do you water plants after a freeze?
You can water plants after a freeze, but it is much more important to make sure they are well-watered before the freeze. Most tissue damage occurs because of a lack of water inside the tissues, which then lose the heat energy from inside the cells (and the cells rupture when they freeze).
Can you kill bacteria by dropping it to a certain temperature?
It depends on the time and temperature. Lets say, you drop the temperature of the bacteria's environment to -50 degrees C for 1 minutes. It would have a larger possibility of making the inner bacterial plasma to freeze and expand thus breaking the cell wall and killing the bacteria then you expose the bacteria to - 100 degrees for 0.001 second. But do be aware of bacterias that have anti-freeze capabilities in their genes. They might stand extreme cold temperature without dying because the anti freeze genes produce anti freeze proteins that help delay the plasma from freezing. But for sure, all beings have their limits eh.... we just don't know the limits are... -RPW-
Do you have to freeze a metal freeze plug?
No, they are just called that since they protect from damage if block freezes.
Where are freeze plugs located?
A freeze plug is located in the side of an engine block that is supposed to protect the block against freeze damage.
How do you stabilize bacteria in liquid form?
It depends what you mean by stabilize. For long term storage of live bacteria, you can add glycerol to the liquid so that the final concentration of glycerol is 15% (I add 1ml of 60% glycerol to 3ml liquid). You can then freeze this without too much of the bacteria being destroyed by ice crystals. It is best to store them at -80ºC if you can, as they will be stable for longer than in a -20ºC freezer. If you just want the bacteria not to grow, then you can transfer them into phosphate buffered saline (PBS) and keep them in the fridge for a couple of days. If you want to 'fix' them, i.e. kill them but maintain their structure long-term, you can transfer them to 4% paraformaldehyde (i.e. 4g paraformaldehyde in 100ml PBS), or 95% ethanol.
To protect crops during the winter farmers spray water on their orange trees to make them freeze in terms of energy gained why does this practice protect the oranges from damage?
when water freezes it releases hidden stored heat which can protect to citrus from freezing
Can you preserve or freeze a bacterial isolate in a normal freezer by just growing them in a broth with glycerol?
Add the sterile glycerol after growing them in broth. However, -80 freezer preserves them for longer than normal freezer.
Where to find non pathogenic freeze dried staph bacteria with growth medium?
Freeze-dried staphylococcus epidermis bacteria (non-pathogenic) where to buy
What happens to becteria when it is refridgerated?
bacteria really dies when you freeze it honestly you cant see bacteria but you know like tap water do have germs such as bacteria so when we freeze or boil the water or other any thing the germs die
Will a car battery be good after it freeze?
If it does freeze, no. The ice would damage the plates/circuitry maybe even the case.
Can a motor run with a missing freeze plug?
Running it without a freeze plug it will run but can and will cause permenant damage to the engine to to the amount of antifreeze you will lose Running it without a freeze plug it will run but can and will cause permenant damage to the engine to to the amount of antifreeze you will lose
What happens to the growth rate of bacteria when you freeze them?
Freezing bacterial cultures slows the death rate of the bacteria. When bacteria is frozen it goes into a sleep state and does not grow or multiply.
Can you freeze chicken twice?
No, you can not freeze chicken twice. Refreezing meats can cause bacteria to start growing and will make you sick when you eat the chicken.
Do you water plants after a freeze?
You can water plants after a freeze, but it is much more important to make sure they are well-watered before the freeze. Most tissue damage occurs because of a lack of water inside the tissues, which then lose the heat energy from inside the cells (and the cells rupture when they freeze).
