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DNA in bacteria is prepared for use in gene transfer by replicating it. When it is transferred, it is already prepared so that it can begin producing new cells based on the provided genetic material.
When genes transfer the tra gene nicks the DNA at its origin of transfer creating a pilus so the chromosome (which contains the gene) can transfer to the other DNA.
DNA is spliced into bacterial DNA in rings called plasmids.
transfer of gene with the help of Ti plasmid present in Agrobacterium which has T-dna region by which gene van transfer.
Genetic Engineering
DNA in bacteria is prepared for use in gene transfer by replicating it. When it is transferred, it is already prepared so that it can begin producing new cells based on the provided genetic material.
When genes transfer the tra gene nicks the DNA at its origin of transfer creating a pilus so the chromosome (which contains the gene) can transfer to the other DNA.
DNA is spliced into bacterial DNA in rings called plasmids.
transfer of gene with the help of Ti plasmid present in Agrobacterium which has T-dna region by which gene van transfer.
The prophage takes an antibiotic resistance gene with it and is packaged with the newly synthesized viral DNA.
a copy of the inserted gene
gene transfer in eukaryotes Gene transfer in eukaryotes may come in the form of mitosis or meiosis. However, horizontal gene transfer, to my knowledge, does not occur in euks. Horizontal gene transfer is when a cell's DNA is transferred to an individual without reproduction. Bacteria have the ability to accept plasmids from other bacteria, and then incorporate the DNA in to their own genome.
Genetic Engineering
Genetics engineering is needed to transfer genes through DNA molecules.
That gene is a functional unit of DNA. And when it is transformed to another organism it carry out that function there.
Bacterial cell wall is made of peptidoglycan(a polymer consisting of both sugars and amino acids). So, when the bacterial cells along with the recombinant DNA is subjected to high temperature and suddenly brought down to low temperature(almost immediately - after 2mins), the protein structure gets denatured. By this, there form pores large enough for the recombinant DNA to enter the cell and express the desired gene when required.
obtain DNA with the desired gene, cut the DNA into pieces, copy the pieces of DNA, identify copies of the desired gene, and transfer the desired gene to the recipient organism. for Plato the answer is A. not B. C. or D.