20 paltelets
The oil immersion lens @100x
750 X times
1000X magnification
The oil immersion lens or objective has power 90X-100X and an eyepiece lens generally in light microscope comes with 10X so total magnification of oil immersion lens is 100X10 = 1,000
1. Focus very carefully with the 40x objective. 2. Rotate turret half way. 3. Apply a small drop of oil. 4. Rotate 100x objective into the immersion.
The oil immersion lens @100x
100x lens is used with oil immersion
oil immersion
The most satisfactory way to study bacterial morphology 40x objective.
100x with oil immersion
The oil immersion lens @100x
Most go up to 1000x as it is light field microscopy. The ocular lens (the one that you look into) is 10x, but there are different objectives to focus on the specimen that you rotate to chose. The lowest is usually 4x, then 10x, 40x, and then 100x. Multiply ocular lens (10x) X objective lens you are using (ex: 40x) = Total 400x Oil immersion drop is used ONLY on the 100x objective.
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
750 X times
1000X magnification
Because that lens (it does not HAVE to be a 100x lens - other lens magnifications are also available in oil immersion constructions) is made to be in contact with a drop of special oil which covers the specimen. The oil has the same index of refraction as the front lens glass and that way a different optical construction can be used.
So that you do not scratch the lens because at 100x magnification it is very very close to the slide.