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I am determining reducing sugar by DNS method to optimize enzyme concentration for rice wine preparation.problem arose when absorbance increase then decrease when enzyme concentration increased.why di?
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Is there a simple equation to calculate the concentration given the absorbance and a time?
You need a graphic concentration versus absorbance.
The absorbance of a solution of NiCl2 of unknown concentration is found to be 0.388 what is the concentration of this soultion?
684
What is the formula that use to calculate the concentration of glucose in blood by the principle of Beer-Lambert law and application of glucose oxidase?
patients absorbance/absorbance of the standard*concentration of the standard gives you the glucose concentration of the patients sample
True or false As the concentration of a colored solution increases the absorbance value decreases?
Absorbance rises with concentration because there is more material for a given frequency of light to be absorbed in. Your statement is false.
Why should the y intersect of any Beer's law plot equal zero?
Beer's law says that absorbance is directly proportional to concentration. So if there is zero concentration there should be zero absorbance (0,0).
Why Beer's law is obeyed at low concentration and not at high concentration?
Because in high concentrations you will have scattering, multi event absorbance, and self-absorbance which are mostly non-linear effects and are not described by the Beer's law.
Is absorbance depends on the temp of the room or solvents in spectrophotometric measurement?
The absorbance depends on the concentration of the chemical species to be analyzed (for identic spectrophotometric cells).
What is absorbance vs concentration?
Absorbance refers to a measure of the capacity associated with a substance as regards absorption of light of a specified wavelength. Whenever you plot a graph of absorbance vs. concentration a direct relationship should be produced
In a plot of absorbance vs concentration what does the slope represent?
The slope of absorbance vs concentration reptresents the value of εb, where ε is the absorbtivity with units of (L/mol cm) and b is path length measured in cm.
How the absorbance data correlates to the initial protein concentration in ug ml?
The absorbance data correlates to the initial protein concentration in ug ml in one way. It breaks down protein in the stomach by the action of the stomach acid.
What is the molar absorption of copper-II sulfate pentahydrate whose absorbance is 0.17 at 650nm?
Depends on the length the light traveled through the solution and the solution concentration. molar absorption = absorbance/(length x concentration) length is typically in cm and concentration is typically in mol/L
If know molar absorbance coefficient of a substance how can beer- Lambert law be used to determine the concentration of a subsatnce?
you would have to know the following values, Absorbance (A), Concentration (C), and cell length (l) and plug it into the formula A=eCl or C = A/Cl
