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If E. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure then will reaction be carried out in waterbath?
Wiki User
∙ 14y ago
no e coli DNA pol can not with stand high temperature if so for every denaturation step we must add fresh enzyme
Wiki User
∙ 14y ago
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Why taq polymerase is thermostable?
Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
Process used to amplify small DNA samples?
I is known as Polymerase Chain Reaction (PCR)
What is PCR short for?
A polymerase chain reaction
Why is a thermostable polymerase used in PCR?
The thermostable polymerase (or Taq polymerase) is a thermostable DNA polymerase (named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965), is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR). Taq polymerase is as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR; Therefore it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75-80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.
What the second step in the Polymerase chain reaction process?
anealing
If e. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure what would happen?
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
What is the source of the polymerase used in polymerase chain reaction?
Taq polymerase, the enzyme used frequently in Polymerase Chain Reaction, is extracted from Thermophilus aquaticus, a thermophilic bacteria.
Why taq polymerase is thermostable?
Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
Which process uses bacteria to copy DNA?
Polymerase chain reaction
Process used to amplify small DNA samples?
I is known as Polymerase Chain Reaction (PCR)
The laboratory procedure for copying selected segments of DNA is?
The laboratory process used to copy specific segments of DNA is called the Polymerase Chain Reaction (or PCR)
What is PCR short for?
A polymerase chain reaction
What does PCR stand for?
Polymerase Chain Reaction
What is the name for the polymerization reaction involving sugars?
carbohydrate polymerase reaction is a condensation polymerization reaction
What is the significance of thermostable polymerase in DNA technology?
It is used in Polymerase Chain Reaction (PCR), which embrace temperature of 94°C - the polymerase has to be able to sustain such temperature.
What is the process in which you bring about a polymerase chain reaction?
To bring about a polymerase chain reaction DNA sequences are placed in .2-.5ml reaction tubes and then placed in a thermal cycler. To achieve the reaction the sequences must undergo 20-40 temperature changes.
What ingredients are needed to start polymerase chain reaction?
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