It is used in Polymerase Chain Reaction (PCR), which embrace temperature of 94°C - the polymerase has to be able to sustain such temperature.
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
Polymerase chain reaction
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
DNA Polymerase.
DNA Polymerase is the enzyme which adds new nucleotides during replication.
to store taq DNA polymerase at low temperature is to make it viable or we can say inactive for sometime. it doesnt die or decay but remains viable.
The thermostable polymerase (or Taq polymerase) is a thermostable DNA polymerase (named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965), is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR). Taq polymerase is as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR; Therefore it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75-80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
Polymerase chain reaction
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
DNA Polymerase.
r DNA technology is technology of creating new combination of DNA. While pcr is one of techniques used in r DNA technology for amplification of perticuler DNA fragment
DNA Polymerase is the enzyme which adds new nucleotides during replication.
PCR is the abbreviation for polymerase chain reaction. It is similar to recombinant DNA technology in that both have the ability to sequence DNA.
DNA polymerase replicated DNA. RNA polymerase creates mRNA to be used in protein synthesis. RNA polymerase does not replicated DNA.
DNA polymerase matches the bases on the parent strand.