r DNA technology is technology of creating new combination of DNA.
While pcr is one of techniques used in r DNA technology for amplification of perticuler DNA fragment
Polymerase chain reaction
PCR is the abbreviation for polymerase chain reaction. It is similar to recombinant DNA technology in that both have the ability to sequence DNA.
It is used in Polymerase Chain Reaction (PCR), which embrace temperature of 94°C - the polymerase has to be able to sustain such temperature.
DNA polymerase-polymerase chain reaction to amplify sections of DNA reverse transcriptase-production of cDNA from mRNA DNA ligase-cutting DNA, creating sticky ends of restriction fragments restriction enzyme-analysis of RFLPs electrophoresis-separation of DNA fragments
Taq polymerase, the enzyme used frequently in Polymerase Chain Reaction, is extracted from Thermophilus aquaticus, a thermophilic bacteria.
I is known as Polymerase Chain Reaction (PCR)
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
Polymerase Chain Reaction
A polymerase chain reaction
In polymerase chain reaction everything is done by temperature with the help of some enzyme. In first step the templet strand is separated by high degree of temperature because in higher temperature the hydrogen bonds between the A-T and G-C are digested.
carbohydrate polymerase reaction is a condensation polymerization reaction
To bring about a polymerase chain reaction DNA sequences are placed in .2-.5ml reaction tubes and then placed in a thermal cycler. To achieve the reaction the sequences must undergo 20-40 temperature changes.