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If you wanted to clone a sequence of DNA - 200 kb long - what vector should you use?
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∙ 14y ago
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∙ 14y ago
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What is a Self replicating DNA used to transmit a gene from one organism to another?
Self replicating DNA used to transmit a gene from one organism to another is a vector. A population of cells carrying a desired plasmid is called a clone.
When did cloning first start?
cloning came from the ancient egiptians saying they wanted to clone each other for their gods, and they wanted their race to be the only ones in the world...
Why do you clone DNA?
Scientists wanted to clone sheep to ultimately learn how to clone a human. But after the failure of cloning the sheep Dolly, many people went against it and cloning against humans is banned in some areas and frowned upon in most.
How do bacteria clone?
bacteria regenerates by cloning, their behaviour will be same and will become smaller, grow again, and again clones, it happens by... in a microscope it will look like a bactiria is been cut into two and will look smaller.
What is a fusion primer?
Not 100% sure, but I believe it's a primer with a linker attached to it. A linker is a short DNA that has a restriction site built-in. Imagine this: you want to amplify a gene by PCR, but it has no suitable restriction site for you to clone into a vector. So by PCR with a fusion primer (with built-in restriction site), you can simply digest the ends and ligate it into your vector
Why is cloning an example of cloning?
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
Why did Michael Jackson want to clone himself?
He never wanted to clone himself.
What is a Self replicating DNA used to transmit a gene from one organism to another?
Self replicating DNA used to transmit a gene from one organism to another is a vector. A population of cells carrying a desired plasmid is called a clone.
Why should scientsist not clone humans?
Scientist should not be able to clone humans because it's to difficult to tell the original human from the clone.
What is pcDNA?
pcDNA is DNA vector used to clone recombinant DNA sequences for the expression of proteins of interest in mammalian cells.
Should you clone people or things?
you should because if you need a transplant, your clone could donate blood or an organ to you.
How would the DNA of your clone compare to the DNA of you?
Technically, it should be identical. The DNA should be exactally the same. This is what makes it a clone.
Should you use phase 1 clone or phase 2 clone?
There is little to no difference.
How do you clone Pokemon in Pokemon Y?
When you're trading with someone, right when it says, please wait after you traded the Pokemon you wanted to clone, turn your game off.
How do i catch three regis in emerald?
get and clone masterballs then do the stuff they tell you to and you should be good get and clone masterballs then do the stuff they tell you to and you should be good
Is it okay to clone farm animals?
NO its not OK to clone animals is it a genetically bonded animal and should not be tampered with x
Significance of vector in DNA recombinant technology?
Vector are plasmid DNA, act as a molecular vehicles to carry genes or DNA of interest. In rDNA technology vectors used to clone the gene by ligation. This chimeric DNA or plasmid can be propagated in E.coli as the vector carries its own origin of replication. Expression plasmid vectors can be used to produce proteins from the gene of interest.
