If you stop at this stage (without the alcohol), the Gram-negative cells will be invisible since they have lost their crystal violet stain.
The gram stain will stain a bacterium then a particular antibiotic might be effective against it. If the gram stain will not stain that particular bacterium, then that particular antibiotic will not be effective against it. Thus a gram negative bacterium like TB requires a different antibiotic than a gram positive bacterium. However, if several have formed a film, it may contain many types and it becomes harder to kill them. That may require a broad based antibiotic.
Because it is a Gram-negative bacterium and can be stained with Gram-negative stain.
Green
purple
You can restain wood with a darker color stain
gram positive Exactly. When doing a gram stain on B. subtilis, this bacterium resists decolorization (keping the first stain and NOT taking on the color of the secondary stain). Therefore, this bacterium is gram (+).
The answer to whether HIV gram-stain positive or negative is that HIV gram-stain is negative. They retain the light red or pink color after the stain.
bacterium that doesn't retain violent stain
The gram stain will stain a bacterium then a particular antibiotic might be effective against it. If the gram stain will not stain that particular bacterium, then that particular antibiotic will not be effective against it. Thus a gram negative bacterium like TB requires a different antibiotic than a gram positive bacterium. However, if several have formed a film, it may contain many types and it becomes harder to kill them. That may require a broad based antibiotic.
Moraxella catarrhalis is a Gram negative bacterium. Thus, after the decolorizer is used, its thin cell wall won't retain the primary stain. The stain is washed away, and Moraxella catarrhalis is colorless.
Saffranin is a " counter stain " . this is also a " basic dye " which is in different color than primary stain ( basic dye crystal violet ) . The purpose counter stain ( saffranin ) is to give the decolourised cells a color , that is different from first one ( crystal violet ). The microbes which are not decolourised by alcohol retains the stain, while the decolourised cell take up the counter stain ( saffranin ).
The Gram stain
Since isopropyl alcohol is colorless, it's not likely to "stain" clothes.However, if the clothes are not completely colorfast, isopropanol is a pretty good solvent, so it may cause the color to fade in the area touched by it. There's also a distant possibility the dye used could react with the isopropanol in such a way as to cause it to change color, but this isn't likely.
Rubbing alcohol all over the stain
You can get color from dye, paint, or stain.
no.
A stain of a contrasting color used to color the components in a microscopic specimen that are not made visible by the principal stain.