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Microbiology How does the charge on a Microorganism affect staining Which staining protocols rely on which charges What are the outcomes?
Maximilian333
The charge of the microorganism is usually negative. The cell membrane is a phospholipid bilayer which is polar, the outside is usually negative. Depending on the staining protocol, a dye will be repelled by or attracted to the cell. Sometimes you use two different dyes- one to color the cell and then one to color the background or surrounding area. Sometimes you want to color the inside of the cell, so you need to melt the cell membrane or cell wall away with heat or chemicals. This allows the dye back in. Sometimes there are steps to resolidify this barrier to trap the dye inside prior to rinsing the slide with water or whatever. These steps are tricky and easy to screw up completely, resulting in a clear or invisible specimen because the dye didn't "take".
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What are the disadvantages of negative staining in microbiology?
It sometimes require additional chemical reagents to produce the desired action.
What are the names of special stains in microbiology?
In microbiology the concept of staining is very important because it highlights the structures of microorganisms allowing them to be seen under a microscope (because ordinarily the microorganisms are somewhat transparent making them difficult to see). In the case of some bacteria, many have specific surface structures such as capsules and flagella as well as internal components such as endospores. To specifically enhance these structures, a special stain may be used. An example of this is using negative staining techniques to see capsules, or using the Ziehl-Neelsen technique to see endospores.
What type of gram is H1N1 bacteria gram-positive or gram-negative?
neither...it is a virus, and gram-staining, a microbiology tool, is not used to help identify or classify a virus
A. bacteria b. viruses c. algae d. protozoa...Which type of microorganism in the pond water would you expect to have its own color so it doesn't need staining?
A.Bacteria
What disease is an acid-fast bacilli smear used for diagnosis?
Acid-fast staining is used in determining tuberculosis. It can also track the progress of antibiotic therapy and determine how contagious a person is. Yay A&M microbiology lab!
What are the disadvantages of negative staining in microbiology?
It sometimes require additional chemical reagents to produce the desired action.
Why was koch's recommemdation for fixing and staining important for microbiology?
Koch's recommendation for fixing and staining is important for microbiology even though all bacteria can be seen under the microscope, because staining and fixing is needed in order to see inside of a transparent bacteria.
What are the names of special stains in microbiology?
In microbiology the concept of staining is very important because it highlights the structures of microorganisms allowing them to be seen under a microscope (because ordinarily the microorganisms are somewhat transparent making them difficult to see). In the case of some bacteria, many have specific surface structures such as capsules and flagella as well as internal components such as endospores. To specifically enhance these structures, a special stain may be used. An example of this is using negative staining techniques to see capsules, or using the Ziehl-Neelsen technique to see endospores.
What clinical specimens are gram stained?
The clinical specimens that are gram stained are microorganism, bodily fluids and sputum specimen. Gram staining makes it easier for the identification of organisms.
What type of gram is H1N1 bacteria gram-positive or gram-negative?
neither...it is a virus, and gram-staining, a microbiology tool, is not used to help identify or classify a virus
A. bacteria b. viruses c. algae d. protozoa...Which type of microorganism in the pond water would you expect to have its own color so it doesn't need staining?
A.Bacteria
What relationship did you find if any between the Gram-staining reaction of a microorganism and its susceptibility to antiseptics and disinfectants?
Gram + bacteria has thick cell walls. This feature makes them more resistant to antiseptic and disinfectants.
What is purity testing of microorganisms?
purity testing of microorganism is to check weahter the microbial culture contain one type of cells of species or it get contaminated or mixed with other type.the best way to check this is by sub culturing and staining.
What is the purpose of smearing in microbiology?
Smear are made for preparing slides for staining which are used in microscopy. The main purpose of smear is to seprate cluster of microbial cells so that we can see them seprately which is helpfull in studying there morphology, and arrangement in colony
What disease is an acid-fast bacilli smear used for diagnosis?
Acid-fast staining is used in determining tuberculosis. It can also track the progress of antibiotic therapy and determine how contagious a person is. Yay A&M microbiology lab!
What is the different staining technique in virology?
1- What_is_the_different_staining_technique_in_virology2- What are the diffrent stain in micro for virus ?
Is staining a material a physical change?
Staining is a chemical process.
