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Oculars are generally 10x, so I assume you're talking of the 40x objective (10x40=400, how total magnification is derived). The most important rule is NEVER touch the coarse focus adjustment, only the fine; you run the risk of breaking the specimen or the objective if you MASH the two together. Other things to consider- high light level, make sure koehler illumination is established and adjust your field aperture to eliminate refringence.

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Q: Proper use of microscope in 400 magnification?
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