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A thioglycollate medium is used for culturing anaerobic bacteria.
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Why will obligate anaerobes grow in thioglycollate?
Obligate anaerobes do not need or use oxygen as a nutrient. In fact it is a toxic substance to them which either kills or inhibits their growth. Thioglycollate allows obligate anaerobes to grow because it consists of sodium thioglycollate, thioglycollic acid and L-crystine which reduce oxygen to water. This creates an anaerobic environment allowing obligate anaerobes to grow.
Where would you expect to see growth of a strict aerobe anaerobe microphile and facultative anaerobe in the fluid thioglycollate medium?
The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.Fluid thioglycollate broth also typically contains a redox potential indicator such resazurin, which produces a pink color in an oxidized environment. As with the BHI media, organisms will only be able to grow where their oxygen requirements are met, and will localize to the area(s) of their oxygen requirements in the fluid thioglycollate broth.Obligate aerobes will grown on or very close to the top (high oxygen). Obligate anaerobes will only grow on the bottom (no oxygen). Facultative anaerobes will grow throughout but more on top. Microaerophiles will be found in a band where only a small amount of oxygen is found. They would be close to but not on the top.
Why must use starch casein nitrate agar and what is the starch casein agar properties?
Because it is a selective medium
What are the ingredients in Brewer's anaerobic agar that remove O2 form the medium?
Oxygen reacts with the molecule sodium thioglycollate. If this is in a broth, the broth will end up having a gradient of available oxygen in it, with lower and lower levels of oxygen the farther down you go to the bottom. As for the brewer's agar, the sodium thioglycollate isn't enough to reduce oxygen levels to a low enough percentage to allow species like Closteridium sporogenes (for example) to grow. These plates must be kept in an airtight chamber with all oxygen somehow removed from it (most commonly a gas pack system that captures oxygen from the air). Hope this helps.
What is basal medium?
A basal medium is a growth medium which incorporates a genetic material such as yeast extract of unknown precise composition.
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
What is the function of thiogllycollic acid in thioglycollate?
It removes trapped oxygen from the medium
How does thioglycollate work?
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
Why you use Fluid Thioglycollate medium for bacterial contamination checking?
Fluid Thioglycollate is a medium used to grow and observe all manner of aerotolerance in bacteria. Therefore if you were to place a sample in it to see if it contained bacteria or not, given the correct temperature needed, the bacteria would be able to be observed fairly quickly as they would grow exponentially in this nutrient rich environment.
When waves travelling from one medium to another medium its speed changeswhy?
The speed of a mechanical wave depends on the mechanical properties of the medium. When the mechanical properties of the medium change, the speed of the mechanical wave changes as a result. The speed of an electromagnetic wave depends on the electrical properties of the medium. When the electrical properties of the medium change, the speed of the electromagnetic wave changes as a result.
Does b.subtilis facultative anaerobes in thioglycollate broth?
No
Why use fresh thioglycollate medium?
A fluid thioglycollate is preferably used soon after preparation to minimize the amount of oxygen that is permitted to diffuse towards the bottom of the medium. The longer you wait before using this medium, the more oxygen is allowed to diffuse into it; more oxygen will negatively affect the growth of obligate anaerobes but would positively affect the growth of aerobic organisms.
What three properties of a medium affect the speed of a sound?
The three properties of a medium that affect the speed of sound are elasticity, density, and temperature. Answered by: Nur _ _ _ _ _ _ Izyani
Why are cysteine and sodium sulfide added to the growth media?
To culture obligate anaerobes, all molecular oxygen must be removed and kept out of the medium. Addition of oxygen-binding agents such as thioglycollate, the amino acid cysteine, or sodium sulfide to the medium prevents oxygen from killing the anaerobes you want to culture.
What are three propertys of a medium affect the speed of sound?
There are many properties. Temperature medium are examples.
Does the speed of a wave depend on the medium it is moving through?
Yes. The speed of mechanical waves, like sound and seismic, depends on the mechanical properties of the medium. The speed of electromagnetic waves, like light and radio, depends on the electrical properties of the medium.
