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What are DNA stuffer regions?

Updated: 10/24/2022
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10y ago

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In a bacteriophage that's been modified as a vector for DNA cloning, a "stuffer region" is a sequence of useless DNA that can be removed with specific endonucleases, to prepare a site for insertion of the DNA to be cloned (sometimes called "passenger DNA" - the idea being that the phage is the vehicle).

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