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i obtain pure culture of bacteria from a mixed culture for obtain pure one bacterial culture
by crystallisation, we can get pure copper sulphate crystals
Streak plating and picking a single colony for the culturing in a broth.
A pure culture is usually derived from a mixed culture (one containing many species) by transferring a small sample into new, sterile growth medium in such a manner as to disperse the individual cells across the medium surface or by thinning the sample manyfold before inoculating the new medium. Both methods separate the individual cells so that, when they multiply, each will form a discrete colony, which may then be used to inoculate more medium, with the assurance that only one type of organism will be present
Solid media is used for the isolation of bacteria as pure culture. 'Agar' is most commonly used to prepare solid media.
Distillation is used frequently.
To determine accuracy
Plates can use more successful methods of pure colony isolation, where single bacterium are separated from one another to allow growth into a visible pure colony for investigation. Also, it is easier to examine bacteria that grow on plates.
It gives microbiologists a way to grow bacteria on a solid medium. This is important if you want to be able to obtain a pure culture, without a solid medium to grow bacteria it would be nearly impossible. On a solid media a single colony can be isolated and used to innoculate a further plate or broth culture. All the bacteria present in a colony should be decendents of a single organism and so be genetically identical. To obtain a pure culture in liquid media, you would have to do numerous serial dilutions which is very labor intensive compared to streaking a solid medium. Even after many serial dilutions there would be no way to ensure a pure culture.
a pure culture is needed when using liquid media as it cannot be used to differentiate a mixed culture.
Agar plate method is used to culture bacteria in lab .
crystallisation is one of the techniques to obtain compounds (solids) in pure form.