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What are methods used to obtain a pure culture?

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Lilliana Rogahn

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Q: What are methods used to obtain a pure culture?
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Why obtain pure culture of bacteria from a mixed culture?

i obtain pure culture of bacteria from a mixed culture for obtain pure one bacterial culture


Which methods is used to obtain pure copper sulphate from impure sample and how?

by crystallisation, we can get pure copper sulphate crystals


What type of culture technique would be used to obtain a pure culture?

Streak plating and picking a single colony for the culturing in a broth.


Describe the methods that you can use to obtain a pure culture and state the significance?

A pure culture is usually derived from a mixed culture (one containing many species) by transferring a small sample into new, sterile growth medium in such a manner as to disperse the individual cells across the medium surface or by thinning the sample manyfold before inoculating the new medium. Both methods separate the individual cells so that, when they multiply, each will form a discrete colony, which may then be used to inoculate more medium, with the assurance that only one type of organism will be present


Which culture medium preparation is most commonly utilized in isolation of pure culture?

Solid media is used for the isolation of bacteria as pure culture. 'Agar' is most commonly used to prepare solid media.


How you can obtain the pure water by which procees distillation or fractional distillation?

Distillation is used frequently.


Why must a pure culture be used in the Kirby-Bauer test?

To determine accuracy


Why are plates used for the culture of microorganisms rather than slopes?

Plates can use more successful methods of pure colony isolation, where single bacterium are separated from one another to allow growth into a visible pure colony for investigation. Also, it is easier to examine bacteria that grow on plates.


What is the value of petri plates in microbiology?

It gives microbiologists a way to grow bacteria on a solid medium. This is important if you want to be able to obtain a pure culture, without a solid medium to grow bacteria it would be nearly impossible. On a solid media a single colony can be isolated and used to innoculate a further plate or broth culture. All the bacteria present in a colony should be decendents of a single organism and so be genetically identical. To obtain a pure culture in liquid media, you would have to do numerous serial dilutions which is very labor intensive compared to streaking a solid medium. Even after many serial dilutions there would be no way to ensure a pure culture.


What is a disadvantage of liquid media?

a pure culture is needed when using liquid media as it cannot be used to differentiate a mixed culture.


What is standard methods agar plates used for?

Agar plate method is used to culture bacteria in lab .


What is crystallisation used for?

crystallisation is one of the techniques to obtain compounds (solids) in pure form.