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What are plasmids and how can they be used to create transgenic organisms?
Wiki User
∙ 11y ago
A plasmid is circular piece of DNA that contains all the genetic information that allows it to bepropagatedinside a living organism. Plasmids are host specific, therefore, a plasmid used in E. coli won't work in yeast. There may be some exceptions.
A plasmid will contain some of these genetic elements.
1. ) Origin of replication - The replication machinery used by the host is compatible with replicating the plasmid as well.
2.) Selection marker - A gene that codes for an enzyme or a protein that givesresistanceto an antibiotic of choice. Kanamycin, ampicillin etc.
3.) MCS - Multi cloning site - DNA sequences which have been specifically designed to contain sites for nucleases so that a gene can be inserted in the right place with the right orientation and transcription frame.
4.) Expression elements - Basically promoters, terminators, ribosome binding sites etc.
If you insert a piece of DNA that the organism didn't have before, it gives that organism some new properties. For example, if the gene codes for a protein that makes it resistant to a number of antibiotics then now you have a new transgenic organism.
That is a poor way of doing it though. More sophisticated way would be to have DNA recombination sites on the plasmid that are homologous with DNA recombination sites on the organism's genome so that the plasmid can beincorporatedinto the genome.
Wiki User
∙ 11y ago
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Why is it essential that the same restriction enzyme be used to create a transgenic organism?
It is essential so that the enzyme does not cut out nucleotides that is not supposed to.
What does plasmids do?
plasmids are vectors that are used to replicate recombinant DNA in a host organism or cell. they are normally used in cloning experiments.
How are transgenic organisms useful in medicine and agriculture?
Transgenic organisms could be used to make a new type of protein. For example, give a bacteria a new gene. The bacteria can then make the protein coded by the new gene. The protein may end being something such as human insulin or some other protein the bacteria don't normally produce. Your welcome Habs 13
What is transgenic organism and recombinant DNA?
a transgenic organism is an organism that has had its genetic code (DNA) altered by the inclusion of one or more genes from another species of organism A transgenic organism is an organism which has been modified with genetic material from another species. The genetic modification is accomplished by inserting DNA into an embryo with the assistance of a virus, a plasmid, or a gene gun. The embryo is allowed to develop, and the mature organism will express the DNA which has been inserted into its genome. Transgenic organisms can also pass the modification on to future generations by breeding with other members of the same species. Genetically altered organisms are used in biological and medical research, production of pharmaceutical drugs, experimental medicine (e.g. gene therapy), and agriculture (e.g. golden rice).
What is the source of most plasmids used in genetic engineering?
plasmids are circular chains of DNA found in bacteria. genetic engineers remove the plasmids out of the bacteria and use enzyme to cut them where they want it cut. after they manipulate the plasmid, they reinsert into the bacteria to observe the changes that were made.The bacteria is usually the source of most of the plasmids that are used in genetic engineering.
What is an example of transgenic organisms?
A transgenic organisms has one or more genes from another organism inserted into its genome.Transgenic bacteria with the gene for human insulin make human insulin that is used to treat people with Diabetes.
What is an example of a transgenic organisms?
A transgenic organisms has one or more genes from another organism inserted into its genome.Transgenic bacteria with the gene for human insulin make human insulin that is used to treat people with Diabetes.
Is prokaryote used in plasmids?
No, it's vise versa. Plasmids are used in and by the prokaryotes.
How are transgenic animals used in the livestock industry?
Currently, they are not. A transgenic animal is one that has been genetically modified to carry a gene that is not normally found in that species. There are a few livestock animals that are transgenic, but they are carefully monitored in research facilities because they are quite expensive to create and to maintain.
Why is it essential that the same restriction enzyme be used to create a transgenic organism?
It is essential so that the enzyme does not cut out nucleotides that is not supposed to.
How is biotechnology used in creating or in the upkeep of a transgenic organism?
Gene therapy is one form of biotechnology which is used in the creation and upkeep of transgenic organisms. Often used in an effort to correct genetic errors such as metabolic errors which cause diseases to be inherited between generations of organisms. Through the use of gene therapy, some of these errors can be corrected and therefore cure or lessen the effects of the genetic defects.
What does plasmids do?
plasmids are vectors that are used to replicate recombinant DNA in a host organism or cell. they are normally used in cloning experiments.
Do mitochondria have plasmids?
No. Plasmids are used for cell replication, and cannot be a fraction of something that a fraction of a cell.
How are transgenic organisms useful in medicine and agriculture?
Transgenic organisms could be used to make a new type of protein. For example, give a bacteria a new gene. The bacteria can then make the protein coded by the new gene. The protein may end being something such as human insulin or some other protein the bacteria don't normally produce. Your welcome Habs 13
What Transgenic bacteria can be used to produce?
Genetically modified bacteria can be used to produce. This is a transgenic bacteria.
What is transgenic organism and recombinant DNA?
a transgenic organism is an organism that has had its genetic code (DNA) altered by the inclusion of one or more genes from another species of organism A transgenic organism is an organism which has been modified with genetic material from another species. The genetic modification is accomplished by inserting DNA into an embryo with the assistance of a virus, a plasmid, or a gene gun. The embryo is allowed to develop, and the mature organism will express the DNA which has been inserted into its genome. Transgenic organisms can also pass the modification on to future generations by breeding with other members of the same species. Genetically altered organisms are used in biological and medical research, production of pharmaceutical drugs, experimental medicine (e.g. gene therapy), and agriculture (e.g. golden rice).
When plasmids are used to transfer foreign DNA to other cells the plasmid is called?
When plasmids, are used to transfer foreign DNA to other cells the plasmid is called a
