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two advantages are you don't need to use radioactive substances and it five accurate results
highly sensitive and specific
not needing radioisotopes (radioactive substances) or a costly radiation counter (a radiation-counting apparatus)
Wiki User
∙ 12y ago
Wiki User
∙ 12y agoit can be difficult to develop an ELISA and the micro titre plates cannot be reused as the Abs bind to the surface and are difficult to remove.
Specificity varies by target; over all very good, but not great specificity
Measures exposure and vaccine response, but not acute infection. For example, once a bird is ELISA Ab positive, the infection is probably over.
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What blot test is used to confirm a seropositive ELISA test for HIV?
The western blot test
What is indirect elisa?
In the Indirect ELISA ,An antigen is added to the microtiter plate well and the antigen attaches to the walls of the microtiter plate.After rinsing to remove excess antigen, the serum suspected of containing the antibodies is added.Enzyme-linked antibody capable of reacting with the constant region of other antibodies is the added, followed by addition of the colorless substrate. Development of color indicates the presence of the antibody being identified.
What does the name Elisa mean?
Elisa means "consecrated to God"
Why is the ELISA test so sensitive?
It has many reason that may be possible. 1. Stability of Elisa kit. 2. Type of Elisa kit ... Example. Rat IL-1 must use with cell rat .... but can use with the other depend cross reactivity of Elisa kit. 3. Right method will show good standard curve. (r2 almost 1 ) if not it's wrong. 4. The concentration used of treated in cell is not appropiate .
What type of test is a ELISA?
an Enzyme Linked immunosorbent assay. during an infection an individual mounts an antibody response which eventually results in production of plasma IgG molecules that bind to various parts of the infectious agent. If these antibodies are present in the sample they will bind to the adsorbed antiagen and remain there after washing and will be detected by the ELISA technique.
The first test given to determine that presence of HIV?
The ELISA test is usually the first test given; quick results and inexpensive. See the related link.
When testing for HIV what tests produce more accurate results than the ELISA test?
Western Blot Test: is a blood test that produces more accurate results than the ELISA test.
What is the difference between direct and indirect ELISA?
Indirect is used to test for ANTIBODIES and direct is used to test for ANTIGENS. For example, Indirect ELISA is used to test for HIV antibodies.
What HIV test produces more accurate results than ELISA test?
There is only one test that can give the accurate results for HIV. The only test that can do this would be the Elisa test.
The confirmatory test for AIDS is?
elisa
What is the agid and elisa test?
The AGID test is the agar gel immunodiffusion test and ELISA is the enzyme-linked immunosorbent assay. Both test for the presence of certain proteins (called antigens) by binding them with antibodies.
What is meant by elisa and its uses?
An Elisa Test is used to test for a substance using antibodies for colour change. The test has been used in medicine and plants. Performing the test involves using an antibody and enzymes.
When testing for HIV a test produces more accurate results than the ELISA test?
Nothing is better then ELISA and CLIA methodology, NAT testing also does not eliminate the window period but only reduces it. The cost is much higher then ELISA updated: western blot test
Use to comfirm a seropositive ELISA test for HIV?
western blot test
What blot test is used to confirm a seropositive ELISA test for HIV?
The western blot test
Which item is from the patient in a direct ELISA test?
antigen
In what year was a blood test to detect HIV founded?
The first test licensed for HIV testing was the ELISA test in 1985. ELISA detects antobodies to the HIV virus, which indicates that the person has been exposed to the virus.
