It has many reason that may be possible. 1. Stability of Elisa kit. 2. Type of Elisa kit ... Example. Rat IL-1 must use with cell rat .... but can use with the other depend cross reactivity of Elisa kit. 3. Right method will show good standard curve. (r2 almost 1 ) if not it's wrong. 4. The concentration used of treated in cell is not appropiate .
It uses an antibody which is specific to the type of antigen/protein you are measuring.
The western blot test
an Enzyme Linked immunosorbent assay. during an infection an individual mounts an antibody response which eventually results in production of plasma IgG molecules that bind to various parts of the infectious agent. If these antibodies are present in the sample they will bind to the adsorbed antiagen and remain there after washing and will be detected by the ELISA technique.
two advantages are you don't need to use radioactive substances and it five accurate results highly sensitive and specific not needing radioisotopes (radioactive substances) or a costly radiation counter (a radiation-counting apparatus)
the most sensitive method for a quantifiable leak test depending on the service of the system.
In the Indirect ELISA ,An antigen is added to the microtiter plate well and the antigen attaches to the walls of the microtiter plate.After rinsing to remove excess antigen, the serum suspected of containing the antibodies is added.Enzyme-linked antibody capable of reacting with the constant region of other antibodies is the added, followed by addition of the colorless substrate. Development of color indicates the presence of the antibody being identified.
The first test performed is ELISA (or EIA) is a test that screens for the presence of HIV antibodies in the blood. This test is not a test for HIV/AIDS its a very sensitive and will be reactive even if there are only 1 or 2 antibodies in that person blood sample. If the results are reactive that test will be followed by a second test called confirmatory test which is highly accurate test in which it's is used to confirm the results of the ELISA.
The ELISA test is usually the first test given; quick results and inexpensive. See the related link.
Western Blot Test: is a blood test that produces more accurate results than the ELISA test.
Indirect is used to test for ANTIBODIES and direct is used to test for ANTIGENS. For example, Indirect ELISA is used to test for HIV antibodies.
There is only one test that can give the accurate results for HIV. The only test that can do this would be the Elisa test.
elisa
The AGID test is the agar gel immunodiffusion test and ELISA is the enzyme-linked immunosorbent assay. Both test for the presence of certain proteins (called antigens) by binding them with antibodies.
An Elisa Test is used to test for a substance using antibodies for colour change. The test has been used in medicine and plants. Performing the test involves using an antibody and enzymes.
Nothing is better then ELISA and CLIA methodology, NAT testing also does not eliminate the window period but only reduces it. The cost is much higher then ELISA updated: western blot test
western blot test
The western blot test
antigen