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some enzyme has degradation of cell membrane &also degrade protein & lipid of the cell....

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12y ago

increase the chemical reactions

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Q: What are the functions of the enzymes in DNA extraction?
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Why are enzymes important to the DNA replication?

Without enzymes, DNA replication would not occur. DNA Polymerases are responsible for catalysing the bonding of nucleotides, and for replacing primers. These functions are essential for DNA replication.


What does sodium citrate do in DNA extraction?

how to make sodium citrate in 10% ethanol for DNA extraction


DNA extraction from bacteria?

DNA extraction from bacteria can be achieved in various ways. Yeast is the best resource to extract the DNA bacteria from using extreme rapid extraction method.


What is DNA extraction?

There are three basic steps in a DNA extraction, the details of which may vary depending on the type of sample and any substances that may interfere with the extraction and subsequent analysis. * Chelate divalent cations such as Mg2+ and Ca2+ to stop dnase enzymes functioning and degrading the DNA * Break open cells by grinding or sonication, and remove membrane lipids by adding a detergent. * Precipitate DNA in cold ethanol or isopropanal, DNA is insoluble in alcohol and clings together; this step also removes salt.


What is the function of CL buffer in DNA extraction?

In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.


List at least four functions of enzymes?

Enzymes serve a wide variety of functions inside living organisms, Enzymes are catalysts that speeds up the digestion of food. Enzymes are responsible for the synthesis of DNA. Enzymes break down starch into glucose, breaks down protein into amino acid, and breaks lipids down into fatty acids.


How do the enzymes pectinase and cellulase aid in the extraction of DNA from strawberries?

In a strawberry to extract the DNA it is required to break down the cell membrane (both the membrane protecting the cell as well as the membrane protecting the nucleus) and pectinase and cellulase in strawberries are enzymes that are break down these membrane...at least in a strawberry.


What is the purpose of Trichloroacetic acid in DNA extraction?

Trichloroacetic acid is used for precipitation of the DNA during its extraction.


What enzymes check for mistakes in replication?

proof reading enzymes, e.g. the exonuclease domain of some DNA polymerases.


Enzymes used to cut DNA molecules in recombinant DNA researsh are?

restriction enzymes


What enzyme do scientists use to cut genes out of strands if DNA?

restriction enzymes


What human functions are enzymes involved in?

In what human functions are enzymes involved