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What does it mean when an antiseptic have a larger zone of inhibition?
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What is the differences between zone of inhibition and zone of exhibition?
Zone of inhibition :-It is the area on an agar plate where growth of a control organism is prevented by an antibiotic usually placed on the agar surface. If the test organism is susceptable to the antibiotic, it will not grow where the antibioitic is. Zone of exhibition:-
What if a agar plate is isolated and antibiotic added and result is no growth or inhibition?
This is basically ANTIBIOTIC SENSITIVITY TEST, to test whether the given organism is RESISTANT(no zone of inhibition) or SENSITIVE( zone of inhibition) to the given antibiotic.Zone of Inhibition Testing is a fast, qualitative means to measure the ability of an antimicrobial agent to inhibit the growth of microorganisms.The effectiveness is based upon the size of zone of inhibition,diffusability of antibiotic,size of inoculum,type of media used.example: bacillus organism is inoculated with both PENICILLIN and AMPICILLIN ,zone of inhibition is absent in case of penicillin and present in case of ampicillin, this shows that ampicillin (sensitive) worked effectively when compared to penicillin.
Does the edge of zone inhibition directly indicate the limit of bacitracin?
Yes, the edge zone of inhibiton directly indicates the limit of bacitracin.
If a petri dish shows a large zone of inhibition what does this say about the antibiotic?
That it is effective, for one thing.
What factors affect the zone of inhibition?
Pathogen susceptibility influences the zone of inhibition because organisms will not grow if they are susceptible to antibiotics. Another influence are the pH levels of agar which should fall between 7.2 and 7.4 room temperature.
Why is a larger zone of inhibition better than a small zone of inhibition?
A larger zone of inhibition means that the applied agent has either inhibited or killed the organisms that were spread on the plate and that those organisms are susceptible to that agent. In other words, a larger zone of inhibition means that the applied agent is more effective in killing/inhibiting the bacteria around it.
How can you determine whether the zone of inhibition is due to death of a bacterium or to inhibition of growth?
How can you determine whether the zone of inhibition is due to death or the effect of the antibiotics?
How to determine whether zone of inhibition is due to death of the bacteria or its inhibition?
If the compound you are testing is bacteriocidal, you will not be able to recover bacteria from the zone of inhibition. If the compound is only bacteriostatic, you should be able to recover bacteria from the zone of inhibition by scraping the surface of the agar and resupending the scrapings in sterile saline and then spreading an aliquot of the suspension on nutrient agar that does not contain the bacteriosttic agent.
What is the differences between zone of inhibition and zone of exhibition?
Zone of inhibition :-It is the area on an agar plate where growth of a control organism is prevented by an antibiotic usually placed on the agar surface. If the test organism is susceptable to the antibiotic, it will not grow where the antibioitic is. Zone of exhibition:-
What if a agar plate is isolated and antibiotic added and result is no growth or inhibition?
This is basically ANTIBIOTIC SENSITIVITY TEST, to test whether the given organism is RESISTANT(no zone of inhibition) or SENSITIVE( zone of inhibition) to the given antibiotic.Zone of Inhibition Testing is a fast, qualitative means to measure the ability of an antimicrobial agent to inhibit the growth of microorganisms.The effectiveness is based upon the size of zone of inhibition,diffusability of antibiotic,size of inoculum,type of media used.example: bacillus organism is inoculated with both PENICILLIN and AMPICILLIN ,zone of inhibition is absent in case of penicillin and present in case of ampicillin, this shows that ampicillin (sensitive) worked effectively when compared to penicillin.
What is the purpose of inoculating tsa plates with samples from the zone of inhibition?
Any bacteria that grow in the zone of inhibition are resistant to the antibacterial used. By inoculating new plates with the bacteria, you will have a pure culture of resistant bacteria.
Why some colony grow inside the zone of inhibition?
mutation that causes resistance
Is antibiotic beyond the zone of inhibition?
the area (zone) in which bacteria cannot grow due to the presence of an antibiotic paper disk
Does the edge of zone inhibition directly indicate the limit of bacitracin?
Yes, the edge zone of inhibiton directly indicates the limit of bacitracin.
If a petri dish shows a large zone of inhibition what does this say about the antibiotic?
That it is effective, for one thing.
What leads to irregular zone of inhibition?
excess liquid on paper disk leads to unequal radial diffusion
What factors affect the zone of inhibition?
Pathogen susceptibility influences the zone of inhibition because organisms will not grow if they are susceptible to antibiotics. Another influence are the pH levels of agar which should fall between 7.2 and 7.4 room temperature.
