Proteins have the higher molecular weight. They consist of long chains of amino acids joined together.
To determine the number of amino acids in your protein, you can use the average molecular weight of an amino acid which is about 110 Da (Daltons). By dividing the molecular weight of your protein (8.9 kD or 8900 Da) by the average weight of an amino acid (110 Da), you can estimate that your protein contains approximately 81 amino acids.
Haha wait are you in Amherst College biochemistry? What are the odds that 682 showed up on another college's problem set? Use 110 g/mol per amino acid (already corrected for the loss of water in amino acid condensation during formation of the protein) and multiply.
In the formation of a peptide bond, a water molecule is "lost" (18Da), so if there are 100 amino acids (of average weight = 128), 99 peptide bonds were created, kicking out 99 x 18 = 162 Da. 100 AA x 128Da = 12,800 Dalton Removing the "water" upon condensation gives 12,800 Da - 162 Da = 12,638 Da = 12,638 g/mol
In terms of molecular weight, proteins are generally heavier than sugars. Proteins are made up of long chains of amino acids, which have higher molecular weights compared to the simpler structures of sugars, which are carbohydrates. For example, a typical amino acid has a molecular weight around 110 daltons, while common sugars like glucose have a molecular weight of about 180 daltons. However, when comparing equal quantities by volume or mass, proteins will typically have a higher weight due to their complex structures.
To calculate the molecular weight of a protein from blood, you typically use techniques like size-exclusion chromatography or SDS-PAGE to separate the proteins based on size. After separation, you can compare the migration distance of the protein of interest with standard proteins of known molecular weights. Additionally, you can use the protein's amino acid sequence, where the molecular weight is calculated by summing the average molecular weights of the individual amino acids and accounting for water molecules released during peptide bond formation. The final molecular weight can be expressed in Daltons (Da).
The human insulin protein is composed of 51 amino acids, and has a molecular mass of 5808 Da. Insulin for other species will be somewhat different.
The average molecular weight of an amino acid is around 110 daltons. However, the molecular weight can vary depending on the specific amino acid due to differences in their side chains.
Yes all the proteins can be theoretically weighed easily by its number of amino acids. An average molecular weight of an amino acid is 110Da. So if a protein is containing 100 amino acids, it would be 100x110=11000Da or 11 kilo Dalton(kDa). Post translational modifications such as glycosylation,phosphorylation, ubiquitination add uo extra mass to the protein.
450
It is not predictable.There are 20 diffent amino acids.
Aequorea GFP is a protein of 238 amino acids with a molecular weight of 27 or 30 kDa. Source: http://www.cryst.bbk.ac.uk/PPS2/projects/jonda/intro.htm
To determine the protein size in kilodaltons (kDa) from the number of amino acids, you can use the average molecular weight of an amino acid, which is approximately 110 daltons. Simply multiply the number of amino acids by 110 to get an estimate of the protein's size in daltons, and then convert the results to kilodaltons by dividing by 1000.