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In the formation of a peptide bond, a water molecule is "lost" (18Da), so if there are 100 amino acids (of average weight = 128), 99 peptide bonds were created, kicking out 99 x 18 = 162 Da. 100 AA x 128Da = 12,800 Dalton Removing the "water" upon condensation gives 12,800 Da - 162 Da = 12,638 Da = 12,638 g/mol
To calculate the extinction coefficient of a protein, you can use the formula: Extinction coefficient (A11cm) / (number of amino acids x molecular weight). A11cm is the absorbance at 280 nm for a 1 cm path length. This value can be determined experimentally using a spectrophotometer.
To determine the number of amino acids in a protein encoded by a DNA molecule, you need to know the specific sequence of nucleotides in the DNA and how many nucleotides correspond to each amino acid. However, if we assume an average molecular weight of 110 daltons per amino acid, you could calculate a rough estimate by dividing the 50 kDa by 110 to get approximately 455 amino acids.
Hydrolysis of a protein would break down the protein into its constituent amino acids. This process involves adding water to the protein molecule, causing the peptide bonds between amino acids to be cleaved, resulting in the release of individual amino acids.
the answer is protein. Kittens97 will be there for you.
Proteins have the higher molecular weight. They consist of long chains of amino acids joined together.
In the formation of a peptide bond, a water molecule is "lost" (18Da), so if there are 100 amino acids (of average weight = 128), 99 peptide bonds were created, kicking out 99 x 18 = 162 Da. 100 AA x 128Da = 12,800 Dalton Removing the "water" upon condensation gives 12,800 Da - 162 Da = 12,638 Da = 12,638 g/mol
The human insulin protein is composed of 51 amino acids, and has a molecular mass of 5808 Da. Insulin for other species will be somewhat different.
To calculate the molecular weight of a protein from blood, you typically use techniques like size-exclusion chromatography or SDS-PAGE to separate the proteins based on size. After separation, you can compare the migration distance of the protein of interest with standard proteins of known molecular weights. Additionally, you can use the protein's amino acid sequence, where the molecular weight is calculated by summing the average molecular weights of the individual amino acids and accounting for water molecules released during peptide bond formation. The final molecular weight can be expressed in Daltons (Da).
Aequorea GFP is a protein of 238 amino acids with a molecular weight of 27 or 30 kDa. Source: http://www.cryst.bbk.ac.uk/PPS2/projects/jonda/intro.htm
It is not predictable.There are 20 diffent amino acids.
Yes all the proteins can be theoretically weighed easily by its number of amino acids. An average molecular weight of an amino acid is 110Da. So if a protein is containing 100 amino acids, it would be 100x110=11000Da or 11 kilo Dalton(kDa). Post translational modifications such as glycosylation,phosphorylation, ubiquitination add uo extra mass to the protein.
Because they are a polymer of amino acids, known as polypeptide (as the amino acids are joined together by peptide bonds). A protein can have hundreds of amino acids, therefore their molecular masses reaches well above 500-800
In terms of molecular weight, proteins are generally heavier than sugars. Proteins are made up of long chains of amino acids, which have higher molecular weights compared to the simpler structures of sugars, which are carbohydrates. For example, a typical amino acid has a molecular weight around 110 daltons, while common sugars like glucose have a molecular weight of about 180 daltons. However, when comparing equal quantities by volume or mass, proteins will typically have a higher weight due to their complex structures.
The molecular weight of butter is approximately 800-1000 g/mol. Butter is a mixture of various fats, with the main components being triglycerides made up of fatty acids, which contribute to its overall molecular weight.
A polypeptide is a chain of amino acids linked by peptide bonds, with a molecular weight of up to about 10,000. It is composed of amino acids.
The approximate molecular weight of palm stearin, which is a mixture of triglycerides derived from palm oil, ranges from 800 to 900 g/mol. This molecular weight can vary depending on the specific composition of fatty acids present in the palm stearin.