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What if the smear you are preparing for staining becomes to dense?
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What is gram variability?
If after staining, a smear reveals both purple(Gram +) and red cells(Gram -). This is generally an indication that the smear contains gram positive cells.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
Function of inoculator loop?
Inoculating loops are for the transferring of microorganisms or for the staining of slides. ?æIt is also called a smear loop or a micro streaker.?æ
What is the purpose of smearing in microbiology?
Smear are made for preparing slides for staining which are used in microscopy. The main purpose of smear is to seprate cluster of microbial cells so that we can see them seprately which is helpfull in studying there morphology, and arrangement in colony
Why dense smear not a good smear preparation?
A dense smear will make it difficult to evaluate cells, as they may be all "piled up" and hard to evaluate.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What is gram variability?
If after staining, a smear reveals both purple(Gram +) and red cells(Gram -). This is generally an indication that the smear contains gram positive cells.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
Why are thick or dense smears less likely to provide a good smear preparation?
because if too much smear the sample will look to indistinct
In gram staining why will very thick smears give inaccurate results?
If a smear exhibits uneven thickness, overlapping cells may not get the proper exposure to the reagents. This results in uneven or mottled staining. For example, in the thicker areas of the smear, gram-negative cells may not decolorize sufficiently and end up staining purple.
Why are spores more difficult to stain than vegetative cells?
Spores are very hard and dense, dye is not readily absorbed into the endospore. However, one method of staining is the Schaeffer and Fulton method. The stain is malachite green and the proper method entails preparing a heat fixed smear which is covered by a piece of blotting paper, and flooded with the dye. Wait 15 mins then remove blotting paper and wash. Counterstain with 0.5% Safrinin. Spores appear green.
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
Function of inoculator loop?
Inoculating loops are for the transferring of microorganisms or for the staining of slides. ?æIt is also called a smear loop or a micro streaker.?æ
Why do you air-dry a smear?
To avoid denaturing and destroying the smear.
