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What is the purpose of heating slide with carbol fuchsin?
to break down the cell wall
Is carbol fuchsin the same as safranin?
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
Difference between hot method and cold method of acid fast staining?
The hot method uses heat to force the primary stain (carbol fuchsin) into the cell wall, while the cold method uses a detergent to cause the stain to penetrate the wall.
What chemical is responsible for the acid fast property of mycobacteria?
the cell wall contains mycolic acid. a dye (carbol fuchsin) is applied to the culture, then washed with acid-alcohol. those cells with mycolic acid in their cell wall will retain the dye even after the alcohol rinse. but those cells without mycolic acid will release the dye.
What ar the component of acid fast staining?
Periodic acid-Schiff (PAS) is a staining method used in histology and pathology. This method is primarily used to identify glycogen in tissues. The reaction of periodic acid selectively oxidizes the glucose residues, creates aldehydes that react with the Schiff reagent and creates a purple-magenta color. A suitable basic stain is often used as a counterstain.Source: http://en.wikipedia.org/wiki/Periodic_acid-Schiff_stain
What is the purpose of heating slide with carbol fuchsin?
to break down the cell wall
Is carbol fuchsin the same as safranin?
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
Name three basic stains?
methylene blue crystal violet carbol fuchsin
What kind of stain do you stain Mycobacterium with?
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
When was Leo Carbol born?
Leo Carbol was born in 1910.
When did Leo Carbol die?
Leo Carbol died in 1991.
Difference between hot method and cold method of acid fast staining?
The hot method uses heat to force the primary stain (carbol fuchsin) into the cell wall, while the cold method uses a detergent to cause the stain to penetrate the wall.
What is the decolorizing agent in the acid fast stain?
The decolorizing agent in the acid fast stain is acid alcohol. The decolorizing agent in the gram stain is ethanol.
Why must heat be used with application of the primary stain during acid fast staining?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
Is carbol fuschin an acidic dye?
No. It is basic. No. It is basic.
How spore staining method works?
Spores are impermeable structures which makes them resistant to dying. Spore staining depends on increasing the permeability of the spore coat by heating to permit the dye in. Upon cooling the dye is trapped inside the spore and not allowed out. The primary dye malachite green is a relatively weakly binding dye to the cell wall and spore wall. In fact, if washed well with water, the dye comes right out of the cell wall, however not from the spore wall once the dye is locked in. That is why there does not need to be a decolorizer in this stain. Upon further treatment with Carbol Fuchsin, the Vegetative cell walls will pick up the counterstain carbol fuchsin and will be stained pink. Spores will be of a light green colour. The identification of spores is very important for the clinical microbiologist who is analyzing a patient's body fluid or tissue since there are not that many spore-forming genera. Spores have two major pathogenic spore-forming genera, Bacillus and Clostridium in which both can cause various types of lethal diseases such as anthrax, botulism, gangrene and tetanus.
