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to break down the cell wall
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∙ 13y ago
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Why should the the slide be flooded with a strong carbol fuschin?
so dat the bacterial sample smear can be mixed with the primary stain throgh heating
What problems arise what the slide is heated in a flame?
Prolonged heating of the slide can cause the slide to shatter.
What is the purpose of prolonged application of heat on the smear?
by application of heat and a mordant, the cell can be stained. the purpose of heating is to soften the waxy material of the cell wall and allow the stain enter the cell. rock on University of Luzon!
Describe the mechanism of action for acid-fast stain?
ACID FAST Os Contains waxlike lipoidal material affecting staining quality. Carbolfuchsin is primary stain. Acid fast organisms resist decolorization with acid alcohol. After decolorization, methyelene blue is added to organisms to counterstain any material that is not acid fast. It is used for examination of Mycobacterium. The principle of staining is depend on the resistance of this type of bacteria to decolarization by acid alcohol, because the cell wall contain waxy material (mycolic acid) which prevent the removal of carbol fuchsin from the cell. Staining Procedure 1. Flood the slide with strong carbol fuchsin and heat until steam rises (but do not boil). 2. After 3-4 min apply more heat until rises again; do not let the stain dry on the slide. 3. About 5-7 min after the first application of heat washes the slide thoroughly under running water. 4. Decolourize in acid-alcohol until all traces of red have disappeared from the film. Decolourization should not be attempted in one stage; there should be intermittent washings in water and re-application of acid-alcohol. 5. Wash well with water and counter stain with Methylene blue for 1 min. 6. Wash and stand end to drain. 7. Acid-fast organisms are red, other organisms are blue.
Why must smear on the slide be completely dry before heat fixation?
Slight heating helps in fixing the cells on to the surface of the glass slide
Why should the the slide be flooded with a strong carbol fuschin?
so dat the bacterial sample smear can be mixed with the primary stain throgh heating
What problems can arise when the slide is heated in a flame?
Prolonged heating of the slide can cause the slide to shatter.
What is the purpose of a point?
Purpose of power point is to create a slide. A slide is a presentation for a business meeting or group.
What is the purpose of the iodine in the second slide of the onion cell?
No purpose
What is gram stain reaction for Borrelia Burgdoferi?
when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
What problems arise what the slide is heated in a flame?
Prolonged heating of the slide can cause the slide to shatter.
What is the purpose of prolonged application of heat on the smear?
by application of heat and a mordant, the cell can be stained. the purpose of heating is to soften the waxy material of the cell wall and allow the stain enter the cell. rock on University of Luzon!
Describe the mechanism of action for acid-fast stain?
ACID FAST Os Contains waxlike lipoidal material affecting staining quality. Carbolfuchsin is primary stain. Acid fast organisms resist decolorization with acid alcohol. After decolorization, methyelene blue is added to organisms to counterstain any material that is not acid fast. It is used for examination of Mycobacterium. The principle of staining is depend on the resistance of this type of bacteria to decolarization by acid alcohol, because the cell wall contain waxy material (mycolic acid) which prevent the removal of carbol fuchsin from the cell. Staining Procedure 1. Flood the slide with strong carbol fuchsin and heat until steam rises (but do not boil). 2. After 3-4 min apply more heat until rises again; do not let the stain dry on the slide. 3. About 5-7 min after the first application of heat washes the slide thoroughly under running water. 4. Decolourize in acid-alcohol until all traces of red have disappeared from the film. Decolourization should not be attempted in one stage; there should be intermittent washings in water and re-application of acid-alcohol. 5. Wash well with water and counter stain with Methylene blue for 1 min. 6. Wash and stand end to drain. 7. Acid-fast organisms are red, other organisms are blue.
What is the purpose of the title slide?
To introduce what it is your about to talk about(:
Why must smear on the slide be completely dry before heat fixation?
Slight heating helps in fixing the cells on to the surface of the glass slide
What is the purpose of slide master?
See you next tuesday
Purpose of fixing the bacterial film?
The purpose of heat fixing the bacteria to the slide is so during the gram staining procedure the bacteria doesn't wash off. If you didn't heat fix the bacteria to the slide, it would not stay on the slide.
