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One method for detecting DNA-protein interaction. EMSA rely on the ability of proteins to bind radiolabels DNA fragments in vitro. Once binding occurs, DNA-protein complexes can be separated from unbound DNA fragments by nondenaturing polyacrylamide gel electroforesis. In general the larger the protein or protein complex bound to the DNA, the greater the extend of retardation of mobility of the radiolabeled DNA fragment. The resulting protein-DNA complexes are visualized by autoradiography.
Methods in muscle Biology, Charles P. Emerson, H. Lee Sweeney.
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What is an assay?
An assaying is a trial by assay, thorough examination and determination.
What is the difference between assay on as is basis and assay on anhydrous basis?
assay on as is basis includes the sample+water content+residual solvents+others. but assay on as is basis takes water content into account that means weight of the water present in the sample will be added in weighing the sample quantity of API required.
What are the common interfering substances for porphobilinogen assay?
The interfering substances for porphobilinogen assay includes urea and urobilinogen. Since they can affect the results of the assay, interfering substances are removed by selectively adsorbing PBG onto an ion-exchange resin (PubMed, 1989).
Why is trichloroacetic acid used in protease assay?
To Stop The Reaction
What is percent RSD limit for assay as per USP?
idotnoww
How does EMSA electromobility shift assay measure whether the protein bends DNA?
EMSA does not measure if protein bends to DNA. It does measure what proteins bind to a specific region of DNA (usually a promoter region). You can use a supershift to determine exactly what protein is binding to the specific DNA region.
What is an assay?
An assaying is a trial by assay, thorough examination and determination.
What is protein assay?
Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
How do you verify gold bar assay certificate?
You can't verify assay certificates.
When was US Assay Commission created?
United States Assay Commission was created in 1792.
When did US Assay Commission end?
United States Assay Commission ended in 1980.
What does the abbreviation DMSA stand for?
The abbreviation D.M.S.A stand for, Dual, Meander, Sleeve, Antenna. Department of Multicultural Sales. DNA Mobility Shift Assay. There's not too much meaning to these corprations.
What is an assay ton?
An assay ton is a standard quantity used in assaying, the act of ascertaining the proportion of a particular metal in an ore or alloy, typically equal to 29.16 grams (short assay ton) or 32.6 grams (long assay ton).
Can you put the word assay in a sentence?
The old miners in gold rush country took their nuggets to the local Assay Office, to determine -- to assay -- the value of the metal in their nuggets.
What is a bandshift assay?
A bandshift assay is a type of assay using gel electrophoresis, in which the mobility of a DNA or RNA probe alone is compared to its mobility in combination with a particular protein.
What is Procedure of assay test of sodium acid citrate?
assay test of sodium acid citrate
Assay of magnesium peroxide in Indian pharmacopoeia?
the sex vidios is very beautiful in assay of magnesium peroxide
