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Increases in the enzymatic activity of some protein kinases important for the regulation of the cell cycle are due to?
the expression of cyclins which pair with specific cyclin-dependent kinases (cdk). Cyclins are expressed at specific times during the cell cycle and regulate the activity of cdk's.
Does total activity increase or decrease during protein purification?
Total Activity almost always decreases during protein purification.
What is a protein used to transport specific substances?
carrier protein
Why does a denature protein no longer function normally?
the function of each protein is a consequence of its specific shape, which is lost when a protein becomes denatured.The shape of a protein determines its specific function within a cell. Denaturing a protein will alter its shape, thus it will no longer function.
Site directed mutagenesis is a molecular biology method used to make what?
Site directed mutagenesis, also called site-specific mutagenesis or oligonucleotide-directed mutagenesis, is used to make specific, intentional changes to the DNA sequence of a gene and/or gene products. It is used to investigate the structure and biological activity of RNA, DNA, and protein molecules. Also used for protein engineering.
How do you calculate specific activity of salivary amylase?
Divide the rate of enzyme activity by the protein concentration.
What is fold purification in protein purification?
A measure of how much more pure your protein is after a purification step in comparison to the crude. You can calculate this by dividing the purified steps specific activity by the crude steps specific activity.
Increases in the enzymatic activity of some protein kinases important for the regulation of the cell cycle are due to?
the expression of cyclins which pair with specific cyclin-dependent kinases (cdk). Cyclins are expressed at specific times during the cell cycle and regulate the activity of cdk's.
What works together to perform a specific function in cell?
The cell organelles work together to carryout living activity in the cell. However, ribosomes receiving message from DNA through mRNA work together to perform specific function of protein synthesis. Receiving specific information to synthesize specific type of protein in the desired amount is the coordinated work of ribosomes.
Does total activity increase or decrease during protein purification?
Total Activity almost always decreases during protein purification.
Why is protein concentration necessary for accurate comparison of the enzyme activity of the fractions?
Enzyme activity sometimes reflects the amount of protein expressed in a cell--however, due to enzyme inhibitors, the enzyme activity is not always reflective of the amount of protein expressed by a cell.
What are proteasomes?
A protein that has enzymatic (ase) activity.That is,it can cleave other proteins (prot) at specific amino acid sequences.Contrast a nuclease, which is a protein that has enzymatic (ase) activity. It is able to cleave nucleic (nuc) acids.All about the prefixes and the suffixes.
What is the protein made in a response to a specific antigen called?
An antigen is a protein made in response to a specific antigen.
What is the medical term meaning a specific protein evoked by an antigen?
An antibody is a specific protein evoked by an antigen.
How do you calculate enzyme enrichment?
Enrichment=specific activity of fraction/original specific activity of original sample
What is RAM protein?
A RAM protein is a protein that only forms a signaling network. Its function is to regulate Ace activity and cellular morphogenesis.
Which end C terminus and N terminus is important to the protein Activity?
To set a target protein in the cell
