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Q: What is contrast as used in microscopy?
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The two most common methods for visualizing live cells are phase-contrast microscopy and differential interference contrast microscopy?

perhaps


What concept of microscopy deals with the ability of the specimen to stand out against its background?

Contrast


What is the difference between phase-contrast microscopy and bright-field microscopy?

Phase-contrast microscopy is the observation of internal structures of living microbes where as bright field microscopy is the observation of killed stained specimens and naturally colored live ones.


What is the importance of contrast in microscopy?

There are many objects that are difficult to see with a microscope. The purpose of the contrast is to make it easier to see specimens that are transparent and unstained.


Which of the following would be best observed for a bacterial cell using phase contrast microscopy?

Motility


Where would phase contrast microscopy would improve image quality?

whenever you're looking at a wet mount you want to use phase contrast


Which type of microscopy is used for colorless specimens?

Transmission electron microscopy


Which works best with phase contrast microscopy wet mount or hang drop?

wet mount slide


What is the definition of the term fluorescence microscopy?

The term "fluorescence microscopy" is a type of light microscopy in which the specimen is irradiated at wavelengths that excite fluorochromes. In medicine, it is used to detect antigens.


What type of slide works best with phase-contrast microscopy?

Hanging drop can be observed for longer periods of time without drying out.


An advantage of fluorescence microscopy over staining techniques is that?

fluorescence microscopy can be used wit any light microscope


Simple staining is often necessary to improve contrast in what microscope?

The type of microscopy that uses chemical stains to add color and increase contrast is light. The type of microscope that can be used to observe very small surface details is called a scanning electron.