hi, could any one answer what is the difference between hplc uv detector and spectrophotometer uv detector ? my id is debendra_mallick2002@yahoomail.com
boh
1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
flourescence is more sensetive than UV detection
We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
the function of a diode detector is to detect stuff
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
Of course.... there isn´t problem....
Detector converts optical signal to electric signal
by injecting the sample in to a HPLC system having PDA detector or Scan the Quetiapine Fumarate sample solution in UV range.