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How do you optimise peak separation in HPLC?
we can optimize peak separation by optimizing the HPLC conditions for standard solutions.
What is single point threshold in peak purity of hplc?
Single point threshold is show only peak maxima spectra whenever peak purity index is shows the front, tail and peak maxima spectra. for pure peak we have to consider three point peak purity which shows that your purity angle should be less than purity threshold which is clearly shows that your peak is specrally pure and homogeneous.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Which one is advanced gc or hplc?
HPLC is advanced
What is C8 and C18?
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
How do you optimise peak separation in HPLC?
we can optimize peak separation by optimizing the HPLC conditions for standard solutions.
Peak-to-valley ratio in HPLC?
to calculate signal to noise use this formulae peak hight *2/noise level
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
How will calculate rrf in hplc?
RRF= (area or height peak X amount IS)/(area or height X amount pk) IS is the internal standard
What is single point threshold in peak purity of hplc?
Single point threshold is show only peak maxima spectra whenever peak purity index is shows the front, tail and peak maxima spectra. for pure peak we have to consider three point peak purity which shows that your purity angle should be less than purity threshold which is clearly shows that your peak is specrally pure and homogeneous.
What is DC offset in signal generator?
A sine wave centered at zero will have a positive peak that is the same magnitude as the negative peak. This can be offset so the negative peak magnitude does not match the positive peak magnitude. For example a 1volt peak - neutral sine wave could be DC offset by 1 volt so the positive peak is at 2 volts and the negative peak is at 0.
How to calculate Relative retention time?
Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Which one is advanced gc or hplc?
HPLC is advanced
What is peak to peak?
Peak to Peak is the most positive peak to the negative peak value. Or find any peak value and multiply by 2.
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What is C8 and C18?
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
