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What is orf in plasmid vector and role in cloning of gene of interest?
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A plasmid a foreign gene and recombinant DNA would be associated with what type of organism?
A plasmid is a double stranded cirucular DNA, used as a vector in cloning. A gene of interest can be ligated into the this to form a chimeric DNA or rDNA. This can be transformed to a bacteria for propagation of the clones (you can amplify them by these transformed bacteria).
What is the role of Cloning Vector in recombinant DNA technology?
The desired gene is cut from its original DNA by using restriction enzyme. The vector (plasmid) is also cut with the same restriction enzyme so that both the vector and the desired gene have the same sticky end. They are bonded together by DNA ligase. Why desired gene are cloned into vector? This is because vector have the ability to reproduce by itself and at the same time carry the desired gene together; in another word, it can replicated the desired gene in the host cell.
What would be the advantages of inserting into a plasmid gene with a highly visible phenotype?
Inserting a plasmid gene into the organism gives us three situation that one is the foreign cell may not pick up the plasmid the second chance is it is picked up may not expressed and in the third case it is expressed and therefore you can have the gene of interest. This is the one main advantage of studying the gene of interest by inserting a plasmid gene.
Why is ampicillin added to the pound medium?
Ampicillin is an antibiotic that is usually used as a reporter gene in cloning. A plasmid containing the ampicillin resistance gene (as well as another target gene within the plasmid) is introduced into the bacterial host. If the bacterium has taken up the plasmid and is expressing the plasmid, it will be resistant to ampicillin. LB is used as a growth medium and ampicillin to verify the plasmid is within the bactrium. No growth means no plasmid in the bacterial host...
What are phage vectors?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
Why is cloning an example of cloning?
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
In the process of human gene cloning using recombinant plasmids what is the bacterial plasmid?
functions as a vector
A plasmid a foreign gene and recombinant DNA would be associated with what type of organism?
A plasmid is a double stranded cirucular DNA, used as a vector in cloning. A gene of interest can be ligated into the this to form a chimeric DNA or rDNA. This can be transformed to a bacteria for propagation of the clones (you can amplify them by these transformed bacteria).
Significance of vector in DNA recombinant technology?
Vector are plasmid DNA, act as a molecular vehicles to carry genes or DNA of interest. In rDNA technology vectors used to clone the gene by ligation. This chimeric DNA or plasmid can be propagated in E.coli as the vector carries its own origin of replication. Expression plasmid vectors can be used to produce proteins from the gene of interest.
What is the role of Cloning Vector in recombinant DNA technology?
The desired gene is cut from its original DNA by using restriction enzyme. The vector (plasmid) is also cut with the same restriction enzyme so that both the vector and the desired gene have the same sticky end. They are bonded together by DNA ligase. Why desired gene are cloned into vector? This is because vector have the ability to reproduce by itself and at the same time carry the desired gene together; in another word, it can replicated the desired gene in the host cell.
What is a vector used for in a genetic engineering experiment?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".
What would be the advantages of inserting into a plasmid gene with a highly visible phenotype?
Inserting a plasmid gene into the organism gives us three situation that one is the foreign cell may not pick up the plasmid the second chance is it is picked up may not expressed and in the third case it is expressed and therefore you can have the gene of interest. This is the one main advantage of studying the gene of interest by inserting a plasmid gene.
Why is ampicillin added to the pound medium?
Ampicillin is an antibiotic that is usually used as a reporter gene in cloning. A plasmid containing the ampicillin resistance gene (as well as another target gene within the plasmid) is introduced into the bacterial host. If the bacterium has taken up the plasmid and is expressing the plasmid, it will be resistant to ampicillin. LB is used as a growth medium and ampicillin to verify the plasmid is within the bactrium. No growth means no plasmid in the bacterial host...
What is the methodology for producing recombinant DNA to be used in gene cloning?
1. A vector such as plasmid is needed along with a host cell. Restriction enzymes and DNA ligase are enzymes that are used to introduce foreign DNA into a vector.
Genetic engineers found a suitable vector to transport a gene from one plant to another in?
the Ti plasmid
What are phage vectors?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
What purpose does restriction mapping serve when it comes to cloning genes?
The enzymes being used to cut the vector open allowing the insertion of the gene of interest. Once the gene has integrated into the host plasmid it can grown in a media conducive to which ever particular strain of bacteria it is. As the transformed bacteria grow in colonies each cell will possesses the gene that was inserted. Now there is a large quantity of "cloned" genes which can be isolated back out and examined. - Rough summary-
