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mgcl2 work as a cofactor and they enhance the enzymatic reaction..
Wiki User
∙ 12y ago
Wiki User
∙ 12y agoMgCl2 is generally used in the composition of Lysis buffer, which is used in the breaking off cell membrane in DNA isolation.
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Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
Role of spooling in blood DNA isolation?
spooling of the DNA results from the addition of the ethanol which is insoluble in the solution. after we get he DNA in the form of spooling structure the solution is centrifuged. so we get the DNA.
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
Why chilled ethanol is added for DNA isolation?
to precipitate extracted DNA
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
What is role of Cetyl trimethyl ammonium bromide in DNA isolation?
it act as as a cationic detergent for the isolation dna from the given sample
Role of chloroform in DNA isolation?
It solubilizes lipids and a lot of proteins to remove them from the DNA.
Role of CTAB in DNA isolation?
It sequester carbohydrates in the solution
What is the role of SE-VEG in DNA isolation?
the role seveg in plant DNA extractions is to remove chlorophyll and similar pigments
Role of phenol chloroform in DNA isolation?
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
Role of additives in PCR?
Reactants: (dNTPs, template DNA (to be amplified), primers(bind to DNA to begin elongation of strand), DNA Polymerase (elongate DNA), & MgCl2) in buffer + H2O
What is role of potassum cloride in Tkm1 buffer for DNA isolation?
for cell lysis
What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?
It is an antioxidant.
Role of magnesium chloride in PCR?
mgcl2 acts as a cofactor for the DNA polymerase used and is necessary for efficient functioning of the pcr
What is the role of sorbitol in DNA isolation?
what is the function of sorbitol in producing plant protoplasts
What is the role of sodium acetate in DNA extraction?
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation
