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What is the difference ods and ods2 HPLC Column?
BDS - Base deactivated Silanol ODS - Octadecyl silane by using the BDS column, residual silanols deacivated and silanol activity reduced, it is end-capped column and it is good for basic compounds. ODS is normal column and high acidic silica, it is not suitable for basic compounds.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Mechanism of end capping in hplc column?
A column is said to be endcapped when a small silylating agent, such as trimethylchlorosilane, is used to bond residual silanol groups (-OH) on a packing surface. It is most often used with reversed-phase packings and may cut down on undesirable adsorption of basic or ionic compounds.
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
What can one read in the HPLC Column?
In an HPLC column one can see very small molecules such as ATP, histidine, glucose, uracil, and pyridine. It is a form high quality of liquid Chromatography.
What is dead volum in hplc?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
What happen after used dipotassium buffer ph 9 on hplc column?
Nothing will happen
What is drift in hplc?
Unstbilized column will deviate baseline as little as possible from a horizontal line
What is mean by delay volume in HPLC analysis?
how long time gradient reach column.
What is the use of calculating asymmetry factor in hplc?
it is used to check the efficiency of the chromatography column used
