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What happens when you leave a stain on the bacterial smear for too long?
Wiki User
∙ 9y ago
If the bacterial cell are under stained then they will loose the stain of dye when wash with alocohol or may be simply by water which then cause a problem in identifying the cell type as e.g. in case of gram staining if the cell do not stain properly with methylene blue then they will loose the stain when washed and will counter stain with saffranin so the gram +ve will show the gram -ve colour.
Wiki User
∙ 15y ago
Wiki User
∙ 9y agoIf you leave the stain on the slide too long the worst that is going to happen is the cells will seem bigger than it really is. If you leave it on not long enough, you wont be able to see cell.
Wiki User
∙ 9y agoThe specimen will?ænot absorb adequate stain, resulting in little contrast. Thus making it difficult for identification of?ædifferent components of the specimen.
Wiki User
∙ 15y agoOver staining the bacterial smear may cause the cell wall distruption or totally destroy the cell wall which results in the loss of true morphological characters of bacterial cell.
Wiki User
∙ 12y agodoesn't stain enough, leaving little contrast
Wiki User
∙ 12y agoLeaving a stain on too long generally stains everything; leaving little contrast.
Leaving a stain on not long enough doesn't stain enough; leaving little contrast.
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What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
This paper will aid in keeping the stain over the bacterial smear.
Why doesn't a negative stain colorize the cells in the smear?
A negative stain will stain the background with an acidic dye, such as Nigrosin. This procedure is used to demonstrate capsules. This technique brings the specimen off of the background for more adequate viewing purposes.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What is the Result of over heating the bacterial smear?
When you heat the bacteria more than three times on the flame of Bunsen burner, the bacteria will damage and if you stain this damaged bacteria, the shape of bacteria is not typical and sometimes you just see the residue of stain on the slide.
Why is it necessary to excessively rinse the smear with water in experiment identifying bacteria using gram stain?
smear should be rinsed with distill water so that all the small particles attach to the smear will washed away such as some time the crystals of dye are attached to the smear which give the illusion of microbial cell some time, distill water is used because it is free from other microbial cell and ions which can harm the smear.
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
This paper will aid in keeping the stain over the bacterial smear.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
Can you leave stain on your wood panels without wiping it off?
Yes, if it's a gel stain. However, if it's a penetrating stain it will not dry correctly. If you try to clean it, it will smear. If you put a clear topcoat on it, the topcoat will smear the excess stain.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
Why should the the slide be flooded with a strong carbol fuschin?
so dat the bacterial sample smear can be mixed with the primary stain throgh heating
What is the consequence of leaving a stain on the bacterial smear too long over staining?
Leaving a stain on too long generally stains everything; leaving little contrast. Leaving a stain on not long enough doesn't stain enough; leaving little contrast.
Why doesn't a negative stain colorize the cells in the smear?
A negative stain will stain the background with an acidic dye, such as Nigrosin. This procedure is used to demonstrate capsules. This technique brings the specimen off of the background for more adequate viewing purposes.
Can you heat fix blood smear before staining for bacterial examination?
You absolutely do not heat fix a blood smear before staining, that is, if you are looking at the blood cells. For bacteria, why wouldn't you culture it first and then heat fix, stain etc. I don't think heat fixing the blood stain would damage the bacterial cells so much as make it hard to differentiate the bacterial cells from the dead, shriveled, ruined blood cells, unless maybe you have like an electron microscope or something.
What is another word for smudge?
Smear
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Green
What is the function of methanol in the wright's stain solution?
to fixed the blood smear
