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cosmids can accept insert fragments of DNA from 30-50 kilobases but plasmids can only accept inserts that are up to 15kb only.
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What is the insert capacity of a cosmid vector in DNA cloning?
The insert capacity of a cosmid vector is about 35-45 kb.
What is the difference between plasmid and episomes?
Plasmid DNA is an extra-chromosomal DNA molecule, it cannot link up with chromosomal DNA, and it contains the genetic informations that are necessary for its own replication. Episomes is any kind of extra-chromosomal DNA that can link up with chromosomal DNA. That is the main difference between them two. Episomes are usually larger than other extra-chromosomal DNA. An example of episome are the viruses, because they intergrate their genetic material into the host's chromosomal DNA.The only difference between the plasmid and the episome is the integration i-e plasmid can not integrate while episome can integrate into the genome.
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
What is the difference between plasmid and vector shall you use all vectors as plasmids?
A vector is a plasmid (usually) that has been engineered to readily accept foreign DNA via recombination. There is also usually special genes previously inserted which code for something that would allow you to distinguish between the colonies which have taken the vector up and which have not, after transformation. A plasmid is the type of DNA baceria usually have - it is circular. There are different names for different sizes and shapes of DNA.
What is a plasmid exchange?
Plasmid Exchange is the transfer of genetic material between bacteria through direct cell to cell contact, or through a bridge-like connection between the two cells.
What are some of the characteristics of cosmid?
A cosmid has the characteristics of a hybrid plasmid. They are used in the building of genomic libraries. They are ~200 base pairs long. More detailed information is available on Wikipedia.
What is a small double stranded DNA that's used as a vector?
it is a ds DNA use in recombinant DNA technology to insert our interested gene and multiply it.Ex;plasmid,cosmid
What is the similarities and difference between the bacteria and human cells?
a human cells have DNA and bacteria has plasmid
What is the insert capacity of a cosmid vector in DNA cloning?
The insert capacity of a cosmid vector is about 35-45 kb.
What is the difference between recombination and transformation?
Recombinants take up the plasmid but do not incorporate it into the DNA. Transformants take up the plasmid and integrate it into the DNA. Recombinants wouldn't express the new genes while Transformants will.
What is the difference between plasmid and episomes?
Plasmid DNA is an extra-chromosomal DNA molecule, it cannot link up with chromosomal DNA, and it contains the genetic informations that are necessary for its own replication. Episomes is any kind of extra-chromosomal DNA that can link up with chromosomal DNA. That is the main difference between them two. Episomes are usually larger than other extra-chromosomal DNA. An example of episome are the viruses, because they intergrate their genetic material into the host's chromosomal DNA.The only difference between the plasmid and the episome is the integration i-e plasmid can not integrate while episome can integrate into the genome.
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.
What is the difference between plasmid and vector shall you use all vectors as plasmids?
A vector is a plasmid (usually) that has been engineered to readily accept foreign DNA via recombination. There is also usually special genes previously inserted which code for something that would allow you to distinguish between the colonies which have taken the vector up and which have not, after transformation. A plasmid is the type of DNA baceria usually have - it is circular. There are different names for different sizes and shapes of DNA.
What is a plasmid exchange?
Plasmid Exchange is the transfer of genetic material between bacteria through direct cell to cell contact, or through a bridge-like connection between the two cells.
What are phage vectors?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
How you clone a gene using cosmid as a vector?
The first step is restriction of the cosmid and the foreign DNA with the restriction enzyme, then ligating the fragments together. Thereafter, the cosmids are loaded into the phage capsid, which leads to the expression of the foreign gene through transduction.
