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magnification.
To locate the exact site in the microscope field that you want to magnify.
Not all microscopes have the same magnification for the lower power lens.
400x
yes it is.
Using high power objective lense
Using high power objective lense
Using high power objective lense
magnification.
To locate the exact site in the microscope field that you want to magnify.
Begin by using the lowest power objective lens and then switching to the next highest power lens.
Not all microscopes have the same magnification for the lower power lens.
This depends entirely on the microscope you are using. Typical high lenses are 150x to 250x, some to 400x.
You use the low power objective lens first to get your sample centered in the field of view.
The fine adjustment knob is used with the power objective on the microscope. Since the objective is already at the highest objective possible for that microscope, using the coarse adjustment knob would focus to much on the object making the details harder to read. Using the fine adjustment knob, you are able to see the fine details within the object or organism you are studying. The higher the power, the finer the knob and the lower the objective, the coarser the knob and objective.
Condenser
First focus the microscope on the lowest objective using first the coarse djustment knob and then the fine adjustment knob. Once you have it in focus, move on and do the same for the next highest objective. Continue this process until you reach the highest objective and focus it in the same fashion.