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Inoculating loops are used to transfer microorganisms to growth media or for staining slides. They are an important part of the sterile technique as their use permits transfer only of the material of interest.
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∙ 16y ago
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∙ 12y agoType your answer here... it is make diluting and spread the micro organism on solid medium by inoculation loop.
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Why heat the inoculating loop after each transfer of microorganisms?
It is necessary to heat the inoculating loop after each transfer in order not to cross contaminate the media you are working with. It is also needed to maintain universal sterile procedures in a laboratory environment.
Function of inoculator loop?
Inoculating loops are for the transferring of microorganisms or for the staining of slides. ?æIt is also called a smear loop or a micro streaker.?æ
Flaming an inoculating loop?
There is no "set" time limit. For proper aseptic technique, you should wait until the inoculating loop/needle is visibly red so that all organisms are dead, etc.
Would a false positive from the reaction between the inoculating loop and hydrogen peroxide be caused by poor specificity or poor sensitivity of the catalase test system?
The false positive from the reaction of hydrogen peroxide and the inoculating loop would be caused by poor specificity. The formula for specificity is TN/TN+FP.
In which direction should you move the inoculating loop in the Bunsen burner flam?
When sterilizing a loop, grasp the handle firmly and begin flaming it starting at the end near the grip, flaming slowly down towards the loop, being sure that the wire is glowing orange. This ensures that the loop is being flamed properly and sterilizing.
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
What are other inoculating instruments aside from needle?
Air gun and inoculating loop are some of the another inoculating instruments. The air gun is used to squirt thin stream of vaccine.
The small amount of growth on the end of an inoculating loop is called an?
Inoculum
Why heat the inoculating loop after each transfer of microorganisms?
It is necessary to heat the inoculating loop after each transfer in order not to cross contaminate the media you are working with. It is also needed to maintain universal sterile procedures in a laboratory environment.
Why should you let the inoculating loop cool first before and after using it?
The inoculating tube is cooled before use so that the organism is not killed by extensive heat.
How inoculating needle use?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
How would you make sure a inoculating loop was sterile?
Pass the loop through a flame 2 or 3 times for about 10 seconds at a time.
What is the most commonly used instrument used for transferring cultures from a broth?
inoculating loop and needle.
Function of inoculator loop?
Inoculating loops are for the transferring of microorganisms or for the staining of slides. ?æIt is also called a smear loop or a micro streaker.?æ
Flaming an inoculating loop?
There is no "set" time limit. For proper aseptic technique, you should wait until the inoculating loop/needle is visibly red so that all organisms are dead, etc.
How will you know when you have flamed the inoculating loop or needle long enough?
when we show the flame in the loop ,for 1 minute its gets brownish colur
In subculturing when do you use the inoculating loop?
Se utiliza una vez el asa de siembra o la aguja se encuentra visiblemente roja, de manera que mueran todos los organismos. De esta manera poder esparcir a su vez la muestra en la laminilla.Translation: It is used once the loop or needle is visibly red, so that all organisms die. Thus turn to spread the sample on the slide.
