I hope this will help you: http://www.sfusd.edu/programs/sf_base/protocols/lab_104_lambda.pdf Fun Fact: DNA stands for Deoxyribonucleic acid (But, you probably already knew that).
restriction endonucleases
they used to cut the DNA at the specific site. For example: BamHI is a restriction enzyme that cuts between the given recognition site:
No. A restriction enzyme cuts DNA when it finds a specific sequence. Different animals will have these sequences occur at different intervals so the length of the fragments won't be the same.
DNA helicase. This is the enzyme that "unzips" DNA.
A restriction enzyme is used to cut DNA (either double or single stranded), cuts can either be "sticky" ( in which cut pieces overlap one another), or "blunt" (in which cut pieces do not overlap). A common rectriction enzyme is EcoR1.
HaeIIIrestriction enzymes
Such an enzyme is called a restriction endonuclease
Restriction enzyme cuts DNA strand at specific locations Restriction enzyme cuts DNA strand at specific locations
A restriction enzyme
A restriction enzyme.
restriction endonucleases
Template Sequence
DNA polymerase :)
Template Sequence
The restriction site is a sequence of DNA that is recognized by an endonuclease, or a protein that cuts DNA, as a site at which the DNA is to be cut. This cutting happens when restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.
they used to cut the DNA at the specific site. For example: BamHI is a restriction enzyme that cuts between the given recognition site:
No. A restriction enzyme cuts DNA when it finds a specific sequence. Different animals will have these sequences occur at different intervals so the length of the fragments won't be the same.