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Heat fixing a smear kills the bacteria with minimal distortion, allows for better staining, and firmly affixes the bacteria to the slide. Chemical fixing is used to preserve fine cellular structures and might stop internal processes in place, protect the cell from damage, or strengthen the cell's structure.
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∙ 9y ago
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∙ 12y agoyou can fix a smear by either heat fixing or chemical fixing.heat fixing preserves the morphological characteristic of the cell while chemical fixing preserves the subcellular characteristics of a cell though it can destroy the enzymatic n protenious contents of such cell.
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∙ 13y agoTake a glass slide and smear [or lay down a thin section of] at least a portion of your/the sample upon the slide. Drying will proceed immediately so to stop this we must apply a fixative agent; thereby af[fix]ing a 'smear'.
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∙ 15y agoThe two methods for fixing a smear are: heat fixation and alcohol fixation.
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∙ 13y agoThe smear is fixed by passing the glass slide over the flame.
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∙ 13y agoSo that during washing with water or treating with alcohol the sample did not flush away.
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∙ 12y agoSumaira sultana
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What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
This paper will aid in keeping the stain over the bacterial smear.
What is the purpose of smearing in microbiology?
Smear are made for preparing slides for staining which are used in microscopy. The main purpose of smear is to seprate cluster of microbial cells so that we can see them seprately which is helpfull in studying there morphology, and arrangement in colony
What are the advantage and disadvantage of heat fixing?
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
Why do you air-dry a smear?
To avoid denaturing and destroying the smear.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What is meant by the term ''fixing the smear''?
Sumaira sultana
Why is it you should heat-fixing the air-dried before staining?
It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.
Why acetone free methanol in lieshmans stain?
methanol for fixing the smear and acetone free to avoid cell membrane lysis
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
This paper will aid in keeping the stain over the bacterial smear.
Why must smear on the slide be completely dry before heat fixation?
Slight heating helps in fixing the cells on to the surface of the glass slide
Can they see if you are pregnant when doing a pap smear?
A pap smear can't detect pregnancy. A pregnancy test is required for that purpose.
What is the purpose of smearing in microbiology?
Smear are made for preparing slides for staining which are used in microscopy. The main purpose of smear is to seprate cluster of microbial cells so that we can see them seprately which is helpfull in studying there morphology, and arrangement in colony
What are the advantage and disadvantage of heat fixing?
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
Why do you air-dry a smear?
To avoid denaturing and destroying the smear.
Do one have to go for a smear after a hysterectomy?
Unless your cervix was removed at the time of your hysterectomy then YES as the purpose of the smear is to find cancerous cervical cells before cancer sets in.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What is the purpose of prolonged application of heat on the smear?
by application of heat and a mordant, the cell can be stained. the purpose of heating is to soften the waxy material of the cell wall and allow the stain enter the cell. rock on University of Luzon!
