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What is the reagents that reacts with crystal violet as the mordant?
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Function of Gram's iodine?
Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
Why do you need a special staining procedure for flagella?
The mordant increases the affinity of cellular components for a dye. Therefore, the mordant allows the silver nitrate to attach strongly to the flagella, so that we can see them under a light microscope.
What is the color when caustic soda reacts with phenolphthalein?
reddish violet
What color is gram negative stain and gram positive stain if you forget to apply iodine?
If iodine is not applied, both the gram-positive and gram-negative stains will appear to be gram-negative. The iodine acts as a mordant that helps to fix the crystal violet stain in the gram-positive bacteria, making them appear purple. Without iodine, the crystal violet stain can be easily washed out of both gram-positive and gram-negative bacteria, resulting in a pink or red color.
Why is Iodine used in Gram staining?
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
What are the reagents and steps min a Gram stain?
The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.
Function of Gram's iodine?
Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.
What is the purpose of mordant in a differential staining procedure?
The resultant crystal violet- iodine (CV-1) complex serves to intensify the-color of the stain. At this point, all cells will appear purple black.
What is the function of iodine solution in the gram stain?
It allows the primary stain crystal violet to remain in the cell instead of being washed out. Due to the larger size of the crystal violet molecule, when the ethanol is applied (the decolorizer) the stain will not be washed out of the Gram (+) positive cells.
What is the purpose of mordant stain in a differential staining procedure?
The resultant crystal violet- iodine (CV-1) complex serves to intensify the-color of the stain. At this point, all cells will appear purple black.
Function of iodine in Gram stain?
iodine act as a mordant..on the gram positive bacteria which got really thick and abundence of peptidoglycan layer, the crystal violet will fix to the peptidoglycan layer..meanwhile in gram negative bacteria which is lack of peptidoglycan layer, the alcohol or acetone will wash it away
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What happens if grams iodine is not applied after staining with crystal violet?
If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.
What is gentian violet?
Traditional antifungal drugs such as.gentian violet (also known as crystal violet)
What is crystal violet iodine complex?
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
What is the extinction coefficient of crystal violet?
balls
