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to remove tannins and polyphenols which are readily present in crude plant extracts

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Q: What is the role of beta mercaptoethanol in DNA extraction?
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What is the role of beta-mercaptoethanol in DNA extraction?

stabilization of phenol against oxidation


What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?

It is an antioxidant.


What role does a cheese cloth play in DNA extraction?

It plays a role.


What is the role of NaCl in DNA precipitation?

roll of Na CL in DNA extraction


Role of phenol chloroform isoamyl alcohol in plasmid DNA extraction?

The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.


What is the role of ethanole in DNA isolation?

2-Mercaptoethanol is often included in extraction buffers designed for plant DNA extraction, because it is a strong reducing agent which can remove tannins and other polyphenols often present in the crude plant extract. It may also help to denature proteins by breaking disulphide bonds between cysteine residues.


What is th role of dnazol in dna extraction?

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Role of saline tris edta in DNA extraction?

Its purpose is to isolate DNA from a protein mixture.


Role of tris in extraction of DNA?

To give the solution buffering capacity.


What is the role of hot water bath in DNA extraction?

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What is the role of mercaptoethanol in DNA isolation?

Mercapotoethanol is used as an antioxidant. Especially for polyphenolics which become insoluble when oxidised and tend to precipitate with the DNA. Sodium sulphite (1.2 g/L) does the same thing without the stink.


Role of beta-mecaptoethanol in DNA isolation?

beta- merceptoethanol denatures the protein by breaking the sulphur bridges in it.