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IN certain cases, enzymes are used to denature (or break up) proteins and disrupt the cell wall (in case of plants). These enzymes require magnesium ions as co-factors in order to catalyze the reaction.
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Role of EDTA in DNA isolation?
it is chealeting agent and has great affinity with metal ions and mg- ions present in dnase as a cofactor and responsible for dnase action that degreded DNA hear edta bide with mg- ions and stop the action of dnase.
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
Role of peg in plasmid DNA isolation?
it reduces the availability of solvents. that is dissociation of H+ ond OH- ions takes place. which makes the macromoleules to condence or crowd. and let to precipitate
What is the function of sodium citrate in DNA isolation?
Sodium ions neutralize the negative charge of the DNA backbone (phosphates) making the DNA less hydrophilic (less likely to be solubilized) in the wash solution
Role of spooling in blood DNA isolation?
spooling of the DNA results from the addition of the ethanol which is insoluble in the solution. after we get he DNA in the form of spooling structure the solution is centrifuged. so we get the DNA.
Role of EDTA in DNA isolation?
it is chealeting agent and has great affinity with metal ions and mg- ions present in dnase as a cofactor and responsible for dnase action that degreded DNA hear edta bide with mg- ions and stop the action of dnase.
What is role of Cetyl trimethyl ammonium bromide in DNA isolation?
it act as as a cationic detergent for the isolation dna from the given sample
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
Role of chloroform in DNA isolation?
It solubilizes lipids and a lot of proteins to remove them from the DNA.
Role of CTAB in DNA isolation?
It sequester carbohydrates in the solution
What is the role of SE-VEG in DNA isolation?
the role seveg in plant DNA extractions is to remove chlorophyll and similar pigments
Role of phenol chloroform in DNA isolation?
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
What is the role of NaCl and edta in DNA isolation?
NaCl provides Na+ ions that will block negative charge from phosphates on DNA. Negatively charged phosphates on DNA cause molecules to repel each other. The Na+ ions will form an ionic bond with the negatively charged phosphates on the DNA, neutralizing the negative charges and allowing the DNA molecules to come together
What is the Role of NaCl in DNA isolation?
NaCl provides Na+ions which form ionic bond with the negatively charged phosphate of DNA,thus neutralizing the effect of negative ,negative repulsion of DNA and helps the DNA molecules to come closer and compact to simplify our process of DNA isolation... BY FARHANA RIYAZ JEZAN UNIVERSITY SAUDI ARABIA.
Role of peg in plasmid DNA isolation?
it reduces the availability of solvents. that is dissociation of H+ ond OH- ions takes place. which makes the macromoleules to condence or crowd. and let to precipitate
What is role of potassum cloride in Tkm1 buffer for DNA isolation?
for cell lysis
What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?
It is an antioxidant.
