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This strain of E. coli has many mutations that make it useful for transformation. Its genotype is
dlacZ DeltaM15 Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rK-mK+) supE44 thi-1 gyrA96 relA1
but the most useful of these mutations are:
The lacZDelta M15 mutation allows for blue/white screening for recombinant cells.
recA1 mutation reduces homologous recombination for a more stable insert.
endA1 mutation reduces endonuclease digestion of plasmid for higher plasmid yield.
hsdR17(rK-mK+) for reduced activity of EcoK restriction enzyme.
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Is dh5 alpha srain of E. coli is dam negative?
no
What type of cells are Ecoli?
Bacteria
Where is ecoli found?
Mostly in urine culture when it is done to diagnose the pt. of urinary tract infection. ecoli is commensol microorganism of intestine.
Which division of bacteria do Ecoli belong and give examples of their human significance?
E.coli belongs to Eubacteria that means true bacteria. They are the key players to digest the food we eat daily. We are more bacteria in our body than our cells combined.
Would detection of EColi in meat be indicative of contamination or spoilage of the product?
Ecoli doesn't develop on food. It reaches food through contamination.
Is dh5 alpha resistant to kanamycin?
no
Is dh5 alpha srain of E. coli is dam negative?
no
What is the significance of E. coli DH5 alpha?
E. Coli DH5 alpha cells are mainly of value to scientific research. They are frequently used for transformation of plasmid DNA, which is a research tool for amplifying the amount of plasmids. One can insert a gene of interest into a plasmid but the yield of this process is limited. If you were then to insert this plasmid into a living cell, your plasmid would be copied with every division of that cell. Bacteria normally host plasmids and are not too picky about it so one more is usually not a problem. Since bacteria like E. coli are really easy to grow, maintain and store, the DH5 alpha strain is perfect. As far as technical details about the strain go: DH5 alpha have been made deficient in some genes, which protects foreign DNA for instance. endA1 gene in DH5 alfa is modified (mutated) so that the intracellular endonuclease it code is inactive that degrades plasmid DNA in many preparations
What is the difference between DH5 alpha and BL21 with reference to cloning?
BL21 is RecA+, whereas DH5alpha is RecA- and thus more appropriate for cloning purposes. BL21 also produces lower levels of proteases and thus better for expression of recombinant proteins.
What and who determines the alpha level in statistics?
Before conducting a significance test, the statistician will choose an alpha level. Depending upon the severity of having type I or type II error, the statistician will make the alpha level higher or lower. Generally in courts, the alpha level is .05. The other common alpha levels for significance tests are .10 and .01.
How does Ecoli poising occur?
When a product has the Ecoli bacteria and you consume it.
Is vancomicyn resistant ecoli contagious and how?
is vancomicyn resistant ecoli contagious
What is the significance of Ecoli ATCC 25922?
This strain is often used for antibiotic susceptibility tests. It's serotype O6, an E. coli EHEC strain that does not produce Shiga toxin.
What is a Alpha Value?
The level of significance; that is the probability that a statistical test will give a false positive error.
Is the ecoli chromosome is longer than the diameter of an individual ecoli bacterium?
yes.
What is the probability of good parst when you have an alpha equal to 0.05 and you will check 100 consecutive parts?
Alpha is the significance level of the test and has little to do with the failure rate of parts (or even parst!).
Is ecoli a psychrophile?
no
