Now this is a benzene ring with a Hydroxide on top, and a bromide on bottom; the p means opposite side
It is used as a pH (acid-base) indicator, particularly for substances with an expected pH between 3.0 and 4.6. Bromophenol blue turns yellow at or below a pH of 3.0. It turns purple at or above a pH of 4.6.
Phenolphthalein Methyl Orange Litmus Bromophenol Blue
Bromophenol blue is the tracking dye in electrophoresis. Being of small molecular size, it races towards the other electrode before the DNA. It is used so that you don't mistakenly let the DNA get washed off the gel and into the buffer solution.
Phenols are acidic substances (pH over 7).
classic recipes say 0.25% bromphenol blue (0.25g/100ml) in a solution containing a viscous substance like: 40%sucrose, or 15%Ficoll, or 30%glycerol all in water. Personally, I use glycerol.
Now this is a benzene ring with a Hydroxide on top, and a bromide on bottom; the p means opposite side
2 bromophenol, 4 bromophenol and hydrogen bromide are formed
blue
Bromophenol Amax or maximum absorbance in spectrophotometer is 590nm I guess so hehe^^ based on my experimental result though!
The loading dye comprises bromophenol blue, Ficoll 400 and water majorly while Xylene cyanol, Tris and EDTA are optional in it. Bromophenol blue is one of the most popular indicators of DNA in agarose gel electrophoresis. Bromophenol blue is a pH indicator.
phenolphthalein
bromophenol blue is used as the tracking dye while coomassie brilliant blue is the staining dye.
Sucrose and Bromophenol blue (6X): 4gm sucrose 25mg bromophenol blue (0.25%) Distilled water to 10 ml
At pH 3.0 it has blue colour
It is used as a pH (acid-base) indicator, particularly for substances with an expected pH between 3.0 and 4.6. Bromophenol blue turns yellow at or below a pH of 3.0. It turns purple at or above a pH of 4.6.
A porch is part of the house. It is the structure in front of the building's entrance.
cause it is sick :p