Want this question answered?
Ninhydrin reacts with proline to give a red colored product
It should react to this reagent. However it must be HEATED before it will react.This is due to the fact that when Ninhydrin is heated it stabilizes and the reacts with the -NH2 groups on the amino acid.
NO, ninhydrin is not the same as super glue
Yes ninhydrin does have flaws. The most common flaw of ninhydrin is the fact that it is so sensitive to the extent of not recording a fingerprint if enough perspiration wasn't created.
Yes. Within "The Chemical Reactions of Amino Acids / Reactions of Amino Groups" there are several examples - one of which is the ninhydrin reaction. A very widely applied reaction of the alpha amino group [that is the N in the peptide bond -CCN-CCN-], it is used to estimate the quantity of amino acids [in a sample] in very small amounts. All amino acids and polypeptides with a free alpha group react with ninhydrin and yield [or produce] an intensely purple colored product - except for Proline and Hydroxyproline {both in which the alpha amino group is termed to be 'substituted' - something to do with carbon rings} which "yield derivatives with a characteristic yellow color." See also 'Schiff's bases'.
I'd have described them as more of a yellowish-orange, myself. Ninhydrin reacts with amino acids. Amino acids are all over your skin. With most of them it forms a yellowish compound; with a couple it's more of the blue-purple you're describing.
Ninhydrin reacts with proline to give a red colored product
Ninhydrin (2,2-dihydroxyindane-1,3-dione) is a substance intended to discover the existence of Ammonia or anything that contains Amines. When this substance reacts with these Amines a blue-purple stain will be observed. Since, the skin contains amino acid, a blue-purple stain will be noticed.
Two.
Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).
It should react to this reagent. However it must be HEATED before it will react.This is due to the fact that when Ninhydrin is heated it stabilizes and the reacts with the -NH2 groups on the amino acid.
It would be the amine group. In fact, ninhydrin will react with other amines, not necessarily those in proteins or amino acids. it reacts with free amines to produce blue-purple color and yellow in proline.
NO, ninhydrin is not the same as super glue
Yes ninhydrin does have flaws. The most common flaw of ninhydrin is the fact that it is so sensitive to the extent of not recording a fingerprint if enough perspiration wasn't created.
Yes. Within "The Chemical Reactions of Amino Acids / Reactions of Amino Groups" there are several examples - one of which is the ninhydrin reaction. A very widely applied reaction of the alpha amino group [that is the N in the peptide bond -CCN-CCN-], it is used to estimate the quantity of amino acids [in a sample] in very small amounts. All amino acids and polypeptides with a free alpha group react with ninhydrin and yield [or produce] an intensely purple colored product - except for Proline and Hydroxyproline {both in which the alpha amino group is termed to be 'substituted' - something to do with carbon rings} which "yield derivatives with a characteristic yellow color." See also 'Schiff's bases'.
Ninhydrin works because it reacts with the amino acids left behind in a latent print. Ninhydrin has a polar carbonyl carbon with is electron deficient. It is attacked by the nucleophilic nitrogren on an amino acid, temporarily combining the ninhydrin and amino acid molecule. The structure is rearranged until the origionally attacked carbon is protonated and leaves in the form of water. This creates a schiff base when the nitrogen is double bonded to the origionally attacked carbon. This molecule rearranges again so that the nitrogen is double bonded to the adjacent carbon of the amino acid. This last rearrangement produces carbon dioxide gas. Further rearrangement of the product produces ruheman's purple.
Well, the best I could come up with is it's either:C9H6O2orC4H3O2If someone knows any different please correct me.jman63: it is actually C9H6O4