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How do you make decolorizer for gram stain?
It is an alchoholic compound i.e. 95% ethanol.
What are two reasons why gram-positive cells may appear pink after the completion of the gram-stain?
If they were old and the cell membrane had degraded, or if you used too much decolorizer.
When did Liquid Liquid end?
Liquid Liquid ended in 1983.
What is the state of matter in which particles are close to each other but do not remain in a fixed position is?
This is the liquid.
Is rain a solid or a gas or a liquid?
Rain is a liquid because it is water.
What color is moraxella catarrhalis after the decolorizer is used?
Moraxella catarrhalis is a Gram negative bacterium. Thus, after the decolorizer is used, its thin cell wall won't retain the primary stain. The stain is washed away, and Moraxella catarrhalis is colorless.
What would the results be if you used the TB decolorizer during the spore stain?
Depends if heat is used
How do you make decolorizer for gram stain?
It is an alchoholic compound i.e. 95% ethanol.
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
What are two reasons why gram-positive cells may appear pink after the completion of the gram-stain?
If they were old and the cell membrane had degraded, or if you used too much decolorizer.
What is the purpose of alcohol used in the gram stain?
The alcohol is a decolorizer. In gram negative organisms, the small amount of peptidoglycan can't hold onto the crystal violet in the presence of the alcohol and so becomes decolorized. The gram positive organisms have a much thicker peptidoglycan layer, and so the crystal violet stays in even with washing by alcohol.
What are the reagents and steps min a Gram stain?
The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.
What are the decolorizer for the fabric pretreatment?
The purpose of the bleaching is to move out the natural pigment of the cotton fiber and make the cotton fabric with necessary white and improve color brightness of dying. The decolorizer have the sodium hypochlorite, sodium chlorite, hydrogen peroxide, peracetic acid, etc.1.sodium hypochlorite bleachingThe sodium hypochlorite is the main reactant of this method. The constitute of the bleaching liquid is similar with the constitute of the sodium hypochlorite, which both should be dechlorinated by the vitriol.2.sodium chlorite bleachingThe sodium chlorite is the main reactant of this method. Pad steaming process bleaching liquid is constitute with the sodium chlorite, activator, buffer agent, surfactant, etc. During the process, it may produce the poisonous chlorine dioxide, which is very harmful to the human.3.hydrogen peroxide bleachingThe hydrogen peroxide is the main reactant of this method. The constitute of the bleaching liquid is similar with the constitute of the hydrogen peroxide. The hydrogen peroxide and peroxidase bleaching is called for, because which are reduce the pollution to the environment.4.peracetic acid bleachingThe peracetic acid is the main reactant of this method. The constitute of the bleaching liquid is similar with the constitute of the peracetic acid. Compared with other bleaching liquid, the peracetic acid have many advantages:(1) oxidation potential is similar to the sodium chlorite (2) don't produce any poisonous, the emission is biodegradable (3) the bleaching is under the weak acid or neutrality condition, which is good for protecting the mechanical property of the fabric. So the peracetic acid bleaching is the best method.By yulong tex
What will be the color of the gram positive if iodine step left out?
Since you've missed the iodine step and you're going to put a decolorizer(alcohol) into it for the next step , naturally it will lost it's color. Because iodine acts as a mordant, which means it helps the gram positive to retain it's color.
How can you remove crystal violet in a Gram Stain?
You won't be able to positively distinguish gram negative from gram positive organisms. Crystal violet is used first so that gram positive organisms will take up the dye and this color is not affected by safrinin later on. Gram negative organisms will lose the purple coloring during the decolorizer step, therefore need to be counterstained so that you can view the organisms.
When was Liquid Liquid created?
Liquid Liquid was created in 1980.
When did Liquid Liquid end?
Liquid Liquid ended in 1983.
