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In Gram positive bacteria it is the cell wall made up of a thick layer of peptidoglycan which holds the stain so that this bacteria can be viewed. Gram negative bacteria have two thin layers of peptidoglycan which loses the stain in the secondary washing, hence their terminology as negative.

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Q: What physical part of bacteria is responsible for the color difference at the end of the gram staining process?
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Related questions

Do gram staining divide bacteria into two groups based on difference in the composition of their DNA?

no. it's their cell walls.


Is staining a material a physical change?

Staining is a chemical process.


What is the purpose of differential staining?

differential staining is a staining technique used to stain colorless bacteria against a dark background.


Is gram staining special staining?

yes it is a special staining like other types....its importance is that one can identify the type of bacteria........


Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?

Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin


What is the advantage over the simple stain?

Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.


What is the advantage of the gram stain over simple stains such as methylene blue?

Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.


What types of organisms are subjected to Negative Staining Technique?

Bacteria .


Why is it important to immobilize the bacteria on a slide prior to staining?

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What staining procedure will be used to observe bacteria in the sputum sample?

acid fast staining or Ziel Neelson staining for observing Mycobacteria tuberculosis or Koch bacilli from sputum sample.


Why is it imortant to immoblize the bacteria on the slide prior to staining?

to kill cells


Why was koch's recommemdation for fixing and staining important for microbiology?

Koch's recommendation for fixing and staining is important for microbiology even though all bacteria can be seen under the microscope, because staining and fixing is needed in order to see inside of a transparent bacteria.