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this depends on whether you are trying to make chemically competant cells or electrically competant cells. the process of making a cell "competant" means that they are capable of accepting 'donor' DNA
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∙ 15y ago
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The host organism into which a cloning vector is placed is called?
Competent cells if i not mistaken
What is the definition of competent cells and how it can help to make a transformation more efficient during the cloning of desired gene?
Generally most of the bacteria have the ability to take up DNA which is present freely in the environment. This is called Competence. It is genetically acquired. But some cells do not exhibit natural competence and they are treated with a suitable chemical like CaCl2 to make their cell wall relatively permeable to DNA. We can make any cell competent in vitro and introduce our desired gene which is taken up by the competent cell. To test the efficiency of our treatment, we may introduce a marker gene like gene for antibiotic resistance such as Tetracyclin onto the desired gene and grow the treated cells on Tetracyclin medium so that only the cells that have taken up the DNA survive.
What is the treatment for precancerous cells?
The treatment options for precancerous cells include cryotherapy, laser therapy, diathermy or cold coagulation. Other treatment options may include cone biopsy or cold coagulation.
What discovery did not contribute to cells theory?
All cells require ATP for energy
What do all the cells that make up your body require?
Electrolytes, their what Cells crave.
Can cells be transformed when they are in a competent state?
They can only be transformed when they are in a competent state.
What is the definition of competent cells?
cells which readily accept a foreign DNA through a process called transformation
How can Dr's refuse medical treatment for serious heart issues?
Any competent patient, including a cardiologist, can choose not to get treatment for any condition.
The host organism into which a cloning vector is placed is called?
Competent cells if i not mistaken
What treatment do serious jaw fractures require?
More serious fractures require complicated, multi-step treatment.
Why do you keep competent cells in ice?
to avoid denaturation of the genetic material in this case DNA
What does Cells require to burn up food?
cells require oxygen to burn sugar.
What is meant by competent cells?
competent cells are the naked DNA(pure DNA without any attached molecules) into a tissue or organ, so the naked DNA is taken up and expressed by some cels and such cells are said to be competent the most common naked DNA vectors are plasmids. i hope that will help you. be a good students, no more cheating lolz
Where is there a drug rehab that specializes in treatment of Oxycontin?
Treatment for all opioid drugs, from Oxycontin to heroin is the same. Any competent treatment center can do it. Look for one that offers in-house Suboxone detox or the equivalent.
How does cancer treatment affect healthy cells?
Cancer treatment affects healthy cells by killing them , because the keno therapy has radiation and it kills your cells.
What types of cells require energy?
All of your cells require energy. Without energy you wouldn't be alive.
What is the definition of competent cells and how it can help to make a transformation more efficient during the cloning of desired gene?
Generally most of the bacteria have the ability to take up DNA which is present freely in the environment. This is called Competence. It is genetically acquired. But some cells do not exhibit natural competence and they are treated with a suitable chemical like CaCl2 to make their cell wall relatively permeable to DNA. We can make any cell competent in vitro and introduce our desired gene which is taken up by the competent cell. To test the efficiency of our treatment, we may introduce a marker gene like gene for antibiotic resistance such as Tetracyclin onto the desired gene and grow the treated cells on Tetracyclin medium so that only the cells that have taken up the DNA survive.
